跳转至内容
Merck
CN
所有图片(1)

文件

安全信息

T0438

Sigma-Aldrich

单克隆抗-转化生长因子-β1 小鼠抗

clone 9016, purified immunoglobulin, lyophilized powder

别名:

抗-TGF-β1, CEDLAP, DPD1, 潜伏期相关肽

登录查看公司和协议定价
所有图片(1)

About This Item

MDL编号:
MFCD00802737
UNSPSC代码:
51111800
NACRES:
NA.41

生物来源

mouse

质量水平

200

偶联物

unconjugated

抗体形式

purified immunoglobulin

抗体产品类型

primary antibodies

克隆

9016, monoclonal

形式

lyophilized powder

种属反应性

mouse, human, rat

技术

ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
neutralization: suitable
western blot: suitable

同位素/亚型

IgG1

UniProt登记号

P01137

应用

research pathology

储存温度

−20°C

靶向翻译后修饰

unmodified

基因信息

human ... TGFB1(7040)
mouse ... Tgfb1(21803)
rat ... Tgfb1(59086)

一般描述

转化生长因子-β1（TGFβ1）被定位到人类染色体19q13.2。
转化生长因子-β（TGF-β）属于分泌蛋白的TGF-β超家族。TGF-β 1以TGF-β1、TGF-β2和TGF-β3三种亚型存在。TGF-β家族参与多种信号通路，涉及丝氨酸/苏氨酸蛋白激酶。TGF-β通过与哺乳动物中的三种跨膜受体（I型，II型和III型受体）结合而起作用。 TGF-β调节的重要通路是SMAD、BMP、MAPK和PI3K/Akt通路。TGF-β 1参与细胞和组织的分化、增殖、炎症、单核细胞、中性粒细胞的迁移和趋化。TGF-β信号传导失调与许多炎症和自身免疫性疾病相关。抗转化生长因子-β1单克隆抗体与人、小鼠和大鼠TGF-β1特异性反应。在ELISA中，该抗体与TGF-β3和TGF-β5的交叉反应性低于2%。

特异性

通过捕获ELISA，观察到与TGF-β3和TGF-β5的<2%交叉反应性。

免疫原

中国仓鼠卵巢细胞系CHO衍生的重组TGF-β1和潜在TGF-β1

应用

推荐浓度：
蛋白质印迹法1 ug/mL（仅在非还原条件下）
免疫组化 3-25 μg/mL
流式细胞术 2.5 μg/106 细胞
ELISA捕获 2-8 μg/mL
ELISA检测 0.1-0.4 μg/mL
中和 0.3-1.0 μg/mL
小鼠源抗转化生长因子-β1单克隆抗体可用于：
  • 免疫组织化学检测平滑肌（2）和肝脏切片中的TGFβ1
  • 脑标本的蛋白质印迹和ELISA
  • 免疫印迹检测巨噬细胞中的TGFβ1

外形

从含碳水化合物的磷酸盐缓冲液中（经0.2 μm过滤）冻干。

重悬

以0.5 mg/mL的浓度在无菌PBS中复溶。

其他说明

从杂交瘤培养上清液中纯化的蛋白A或G。

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

Not finding the right product?  

Try our 产品选型工具.

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

常规特殊物品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

  1. How does the storage temperature relate to shipping conditions?

    The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. See Shipping and Storage for more information.

  2. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  3. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  4. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  5. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  6. Can Product T0438, Monoclonal Anti-Transforming Growth Factor-?1 antibody produced in mouse, be used to neutralize the biological activity of TGF-ß1?

    Yes, this antibody will neutralize TGF-ß1.  

  7. Can Product T0438, Monoclonal Anti-Transforming Growth Factor-?1 antibody produced in mouse, be used for immunohistochemistry with frozen and/or formalin-fixed paraffin-embedded sections?

    This antibody can be used with both formalin-fixed paraffin-embedded (FFPE) tissue sections and frozen sections.

  8. Will Product T0438, Monoclonal Anti-Transforming Growth Factor-?1 antibody produced in mouse, work for intracellular flow cytometry?

    This antibody can be used for intracellular flow cytometry. The cells should be fixed with 4% paraformaldehyde and permeabilized with a 0.1% saponin-containing buffer.

  9. How is Product T0438, Monoclonal Anti-Transforming Growth Factor-?1 antibody produced in mouse, different than T0688,  Anti-Transforming Growth Factor-?1 antibody produced in chicken?

    These two antibodies are very similar in regards to the applications they are suitable for. They do differ in a few key areas. T0688 only recognizes active TGF-ß1 while T0438 recognizes both active and latent TGF-ß1. Also, T0438 is a mouse monoclonal antibody and T0688 is a chicken polyclonal antibody.

  10. What is the molecular weight of Product T0438, Monoclonal Anti-Transforming Growth Factor-?1 antibody produced in mouse,?

    TGF-ß1 is a disulfide-linked homodimer of two 12.5 kDa subunits. Under non-reducing conditions, the T0438 antibody should detect a protein at 25 kDa.

  11. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Alba Di Pardo et al.
Molecular brain, 6, 55-55 (2013-12-18)
Huntington Disease (HD) is a neurodegenerative disorder resulting from the expansion of polyglutamine stretch in the huntingtin protein (Htt). Mutant HTT (mHtt) leads to progressive impairment of several molecular pathways that have been linked to disease pathogenesis. Defects in the
The potential antifibrotic impact of apocynin and alpha-lipoic acid in concanavalin A-induced liver fibrosis in rats: Role of NADPH oxidases 1 and 4.
Fayed MR, et al.
Drug Discoveries & Therapeutics, 12(2), 58-67 (2018)
Gangwen Han et al.
International journal of biological sciences, 8(2), 228-235 (2012-01-19)
TGFβ1 was initially identified as a potent chemotactic cytokine to initiate inflammation, but the autoimmune phenotype seen in TGFβ1 knockout mice reversed the dogma of TGFβ1 being a pro-inflammatory cytokine to predominantly an immune suppressor. The discovery of the role
The transforming growth factor-beta1 (TGFB1) gene is associated with chronic obstructive pulmonary disease (COPD).
Celedon JC, et al.
Human Molecular Genetics, 13(15), 1649-1656 (2004)
Changes of peripheral TGF-beta1 depend on monocytes-derived macrophages in Huntington disease.
Di Pardo A, et al.
Molecular Brain, 6(1), 55-55 (2013)
查看所有相关文献

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门