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安全信息

STS0033

Sigma-Aldrich

Agarose tablets

Low EEO, For molecular biology (200 x 500mg tablets)

别名:

3,6-Anhydro-α-L-galacto-β-D-galactan, Agarose LE, Agarose tablets

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About This Item

CAS号:
9012-36-6
MDL编号:
MFCD00081294
UNSPSC代码:
41105317
NACRES:
NB.21

生物来源

algae (marine)

等级

for molecular biology

表单

tablet

杂质

≤10.0% moisture content

颜色

white

EEO

0.09-0.13 (-Mr)

转变温度

transition temp 34.5-37.5 °C

凝胶强度

≥1200 g/cm2 (1% gel)

痕量阴离子

sulfate (SO42-): ≤0.15%

适用性

suitable for electrophoresis
suitable for molecular biology

异质活性

DNase, RNase, none detected

SMILES字符串

O1[C@H]([C@@H]([C@H]([C@H]([C@H]1CO)O)O[C@@H]4O[C@@H]5[C@H]([C@@H](OC5)[C@@H]4O)O[C@@H]6O[C@@H]([C@@H]([C@@H]([C@H]6O)O)O)CO)O)O[C@H]2[C@H]3OC[C@@H]2O[C@H]([C@H]3O)O

InChI

1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1

InChI key

MJQHZNBUODTQTK-WKGBVCLCSA-N

相关类别

一般描述

Agarose is commonly used in research for gel electrophoresis to separate DNA and RNA fragments. It is a natural polysaccharide isolated and purified from red algae. It has a linear structure consisting of alternating D-galactose and 3,6-anhydro-L-galactose units. When dissolved in solution, upon cooling, it forms a solid yet porous gel to use as a medium to separate nucleic acid fragments by size. When an electrical current is applied the charged molecules migrate through the gel.

应用

Agarose tablets can be used in research applications for:



  • Separation and analysis of nucleic acids by agarose gel electrophoresis, Northern Blot, or Southern Blot
  • Single Cell Gel Electrophoresis (SCGE), also known as comet assays
  • Hydrocolloid gels to study the structural influence of gels on the release of carbohydrates
  • Ouchterlony double immunodiffusion plates used to quantify antibodies and antigens
  • Radial immunodiffusion (RID)

特点和优势

  • Qualified for molecular biology applications
  • Free of endonucleases: DNase and RNase
  • Convenient, quick and easy to use
  • Low EEO and gelling temperature for routine analysis
  • High gel strength 1,200 g/cm2 (1% gel)
  • Separates DNA & RNA fragments 100bp - 23kb in 2% - 0.5% agarose gel, respectively.
  • Northern or Southern blotting, preferred for fragment sizes >1kb

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说明
价格

储存分类代码

11 - Combustible Solids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

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