SRP7783
MMP-3 人
recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC)
别名:
EC 3, Matrix metalloproteinase-3, Stromelysin-1
产品名称
MMP-3 人, recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC)
biological source
human
recombinant
expressed in E. coli
assay
≥95% (HPLC)
≥95% (SDS-PAGE)
form
lyophilized
mol wt
~19.5 kDa
packaging
pkg of 10 μg
technique(s)
western blot (chemiluminescent): suitable
impurities
endotoxin, tested
NCBI accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... MMP3(4314)
Application
MCP-1 has been used to induce chemotaxis in mice macrophages.
MMP-3 human has been used to develop a simple and sensitive electrogenerated chemiluminescence (electrochemiluminescence (ECL)) peptide-based bioassay for the detection of matrix metalloproteinases releasing from living cells.
Biochem/physiol Actions
Matrix metallopeptidase 3 (MMP3) has a broad substrate specificity and is capable of degrading fibronectin, laminin, collagens III, IV, IX, and X, and cartilage proteoglycans. It participates in wound repair, progression of atherosclerosis, tissue remodeling and tumor initiation. MMP3 variants can be associated with hypertrophy of interventricular septum or hypertrophic cardiomyopathy.
The gene JE, also referred to as MCP-1 (monocyte chemoattractant protein-1), encodes a secreted chemokine that functions as a chemoattractant for monocytes and may activate their anti-tumor properties. This protein may serve as a potential therapeutic anticancer agent.
General description
Active MMP-3 catalytic domain from human cDNA, expressed in E. coli and purified using proprietary technologies. The active MMP-3 is very useful in studying enzyme kinetics, cleave target substrates, and screen for inhibitors.
The gene JE is an early growth response gene that was first identified in mouse 3T3 cells. The gene is mapped to mouse chromosome 11. It encodes a member of the CC subfamily, the members of which contain characteristic two adjacent cysteine residues.
Physical form
Lyophilized powder.
Preparation Note
Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.
Reconstitute in sterile PBS to a concentration of 0.1-1.0 mg/mL. This solution can then be diluted into other aqueous buffers.
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Suppression of tumor formation in vivo by expression of the JE gene in malignant cells.
Rollins BJ and Sunday ME
Molecular and Cellular Biology, 11, 3125-3131 (1991)
Simple and sensitive electrogenerated chemiluminescence peptide-based biosensor for detection of matrix metalloproteinase 2 released from living cells
Dang Q, et al.
Analytical and Bioanalytical Chemistry, 408(25), 7067-7075 (2016)
Rowan Pentz et al.
Neurobiology of disease, 148, 105150-105150 (2020-11-02)
Matrix metalloproteinase-3 (MMP-3) has been associated with risk of Alzheimer's disease (AD). In this study we introduce a novel role for MMP-3 in degrading nerve growth factor (NGF) in vivo and examine its mRNA and protein expression across the continuum
Grzegorz Wiera et al.
Cellular and molecular life sciences : CMLS, 78(5), 2279-2298 (2020-09-23)
Learning and memory are known to depend on synaptic plasticity. Whereas the involvement of plastic changes at excitatory synapses is well established, plasticity mechanisms at inhibitory synapses only start to be discovered. Extracellular proteolysis is known to be a key
Hematopoietic-Derived Galectin-3 Causes Cellular and Systemic Insulin Resistance.
Li P
Cell, 167, 973-984 (2016)
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