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生物来源
human
重组
expressed in E. coli
方案
≥95% (HPLC)
≥95% (SDS-PAGE)
表单
lyophilized
分子量
~19.5 kDa
包装
pkg of 10 μg
技术
western blot (chemiluminescent): suitable
杂质
endotoxin, tested
NCBI登记号
运输
wet ice
储存温度
−20°C
基因信息
human ... MMP3(4314)
一般描述
Active MMP-3 catalytic domain from human cDNA, expressed in E. coli and purified using proprietary technologies. The active MMP-3 is very useful in studying enzyme kinetics, cleave target substrates, and screen for inhibitors.
The gene JE is an early growth response gene that was first identified in mouse 3T3 cells. The gene is mapped to mouse chromosome 11. It encodes a member of the CC subfamily, the members of which contain characteristic two adjacent cysteine residues.
应用
MCP-1 has been used to induce chemotaxis in mice macrophages.
MMP-3 human has been used to develop a simple and sensitive electrogenerated chemiluminescence (electrochemiluminescence (ECL)) peptide-based bioassay for the detection of matrix metalloproteinases releasing from living cells.
生化/生理作用
Matrix metallopeptidase 3 (MMP3) has a broad substrate specificity and is capable of degrading fibronectin, laminin, collagens III, IV, IX, and X, and cartilage proteoglycans. It participates in wound repair, progression of atherosclerosis, tissue remodeling and tumor initiation. MMP3 variants can be associated with hypertrophy of interventricular septum or hypertrophic cardiomyopathy.
The gene JE, also referred to as MCP-1 (monocyte chemoattractant protein-1), encodes a secreted chemokine that functions as a chemoattractant for monocytes and may activate their anti-tumor properties. This protein may serve as a potential therapeutic anticancer agent.
外形
Lyophilized powder.
重悬
Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.
Reconstitute in sterile PBS to a concentration of 0.1-1.0 mg/mL. This solution can then be diluted into other aqueous buffers.
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
历史批次信息供参考:
Simple and sensitive electrogenerated chemiluminescence peptide-based biosensor for detection of matrix metalloproteinase 2 released from living cells
Dang Q, et al.
Analytical and Bioanalytical Chemistry, 408(25), 7067-7075 (2016)
Rowan Pentz et al.
Neurobiology of disease, 148, 105150-105150 (2020-11-02)
Matrix metalloproteinase-3 (MMP-3) has been associated with risk of Alzheimer's disease (AD). In this study we introduce a novel role for MMP-3 in degrading nerve growth factor (NGF) in vivo and examine its mRNA and protein expression across the continuum
Grzegorz Wiera et al.
Cellular and molecular life sciences : CMLS, 78(5), 2279-2298 (2020-09-23)
Learning and memory are known to depend on synaptic plasticity. Whereas the involvement of plastic changes at excitatory synapses is well established, plasticity mechanisms at inhibitory synapses only start to be discovered. Extracellular proteolysis is known to be a key
J S Frieling et al.
Oncogene, 36(31), 4498-4507 (2017-04-04)
Parathyroid hormone-related protein (PTHrP) is a critical regulator of bone resorption and augments osteolysis in skeletal malignancies. Here we report that the mature PTHrP
E V Privalova et al.
Kardiologiia, 54(5), 4-7 (2014-09-02)
Prognosis of patients with hypertrophic cardiomyopathy (HCMP) to a great extent is determined by clinical variant of the disease. As the system of matrix metalloproteinases (MMPs) plays an important role in development and progression of the processes of fibroformation and
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