SRP6458
TFPI 人
recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)
别名:
EPI, LACI, TFI, TFPI1
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关于此项目
NACRES:
NA.32
UNSPSC Code:
12352202
Biological source:
human
Recombinant:
expressed in HEK 293 cells
Assay:
≥95% (SDS-PAGE)
Form:
lyophilized
Mol wt:
calculated mol wt 30 kDa, observed mol wt 41-45 kDa (DTT-reduced. Protein migrates due to glycosylation. Asp 29 is the predicted N-terminal.)
Impurities:
<1 EU/μg endotoxin (LAL test)
biological source
human
recombinant
expressed in HEK 293 cells
tag
6-His tagged (C-terminus)
assay
≥95% (SDS-PAGE)
form
lyophilized
potency
<0.35 nM IC50
mol wt
calculated mol wt 30 kDa, observed mol wt 41-45 kDa (DTT-reduced. Protein migrates due to glycosylation. Asp 29 is the predicted N-terminal.)
packaging
pkg of 10 μg
impurities
<1 EU/μg endotoxin (LAL test)
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... TFPI(7035)
General description
TFPI（组织因子途径抑制物）基因定位于人染色体 2q32.1。该蛋白质以两种同工型（TFPIα 和 TFPIβ）存在。
组织因子途径抑制物（TFPI）也称为外源通路抑制因子（EPI）、脂蛋白相关凝结抑制剂（LACI），是由血管内皮细胞合成的血浆蛋白酶抑制因子，它的部分与这些细胞的糖胺聚糖结合。TFPI 是一种单链多肽，可以可逆地抑制因子 Xa（Xa）和凝血酶（因子 IIa）。TFPI 是一种分泌的蛋白质，具有一个 N 末端酸性区，三个被两个连接子隔开的 Kunitz（K）结构域和一个 C 末端碱性区。第一个 K 结构域抑制凝血因子 VIIa 与组织因子（TF）复合；第二个 K 结构域抑制因子 Xa；第三个 K 结构域与肝素结合；C 末端碱性区可能具有多种功能。例如，它在 TFPI 与细胞表面结合中起重要作用。
Biochem/physiol Actions
TFPI（组织因子途径抑制物）抑制组织因子功能和血栓形成是颈动脉疾病缺血性卒中的主要原因。TFPI 的主要功能是调节凝血系统。它具有抗凝血和抗转移作用。该蛋白显著地定位于动脉粥样硬化斑块的中膜和新生内膜平滑肌细胞、巨噬细胞和 T 细胞。在动脉粥样硬化中观察到 TFPI 基因的上调。TFPI 基因突变会导致患冠心病的风险。
Physical form
从 0.22 μm 过滤的 PBS 溶液（pH 7.4）中冻干。通常在冻干前加入甘露醇或海藻糖作为保护剂。
Preparation Note
打开样品瓶之前需离心。用无菌的PBS，pH7.4重悬成浓度为50 μg/mL的溶液。不可涡旋。该溶液可在2-8°C下储存长达1个月。如要长期储存，建议将其存放于-20°C。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Significant genetic association of a functional TFPI variant with circulating fibrinogen levels and coronary artery disease
Naji DH, et al.
Molecular Genetics and Genomics, 293(1), 119-128 (2018)
Teresa C Assumpção et al.
PLoS neglected tropical diseases, 10(1), e0004298-e0004298 (2016-01-09)
Hematophagous mosquitos and ticks avoid host hemostatic system through expression of enzyme inhibitors targeting proteolytic reactions of the coagulation and complement cascades. While most inhibitors characterized to date were found in the salivary glands, relatively few others have been identified
Increased expression of TFPI in human carotid stenosis
Stavik B, et al.
Thrombosis Research, 155(5), 31-37 (2017)
John W Avery et al.
PloS one, 7(2), e31090-e31090 (2012-02-22)
Low birth weight and fetal loss are commonly attributed to malaria in endemic areas, but the cellular and molecular mechanisms that underlie these poor birth outcomes are incompletely understood. Increasing evidence suggests that dysregulated hemostasis is important in malaria pathogenesis
Harald Haidl et al.
Scientific reports, 9(1), 8014-8014 (2019-05-31)
Healthy neonates exhibit a well-functioning haemostatic system despite peculiarities regarding composition of clotting factors and inhibitors as well as impaired platelet aggregation. Thrombocytopenia and severe bleeding events are feared in sick infants. Recombinant factor VIIa (rFVIIa) is a haemostatic agent
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