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生物来源
human
重组
expressed in HEK 293 cells
标签
6-His tagged (C-terminus)
方案
≥95% (SDS-PAGE)
表单
lyophilized powder
分子量
calculated mol wt 45 kDa
observed mol wt 18 kDa
observed mol wt 32 kDa
observed mol wt 50 kDa
包装
pkg of 10 μg
pkg of 50 μg
杂质
<1 EU/μg endotoxin (LAL test)
UniProt登记号
运输
wet ice
储存温度
−20°C
基因信息
human ... uPA(5328)
一般描述
PLAU（纤溶酶原激活物，尿激酶）基因定位于人类染色体 10q22.2
尿激酶型纤溶酶原激活物又称 PLAU 和 uPA。人 uPA 最初合成为 431 个氨基酸前体，N 端信号肽（20 个残基）。单链分子被加工成不同分子量的二硫键连接的双链分子。从 Ser21（长型）和 Lys156（短型）开始，A 链以两种形式存在。长、短 A 链分别为高、低分子量形式所特有。长 A 链包含一个负责结合 PLAU 受体的EGF（表皮生长因子）样结构域。B 链对应于催化域。编码它的基因定位于10号人类染色体。
DTT-reduced. Protein migrates as three bands corresponding to the long α chain, β chain and unprocessed full-length chain due to glycosylation and cleavage. Ser21, Ile179 & Lys156 is the predicted N-terminus.
生化/生理作用
尿激酶型纤溶酶原激活物是一种底物特异性极其有限的丝氨酸蛋白酶，其裂解纤溶酶原中的 Cys-Pro-Gly-Arg560-Val561-Val-Gly-Gly-Cys 序列从而形成纤溶酶。在与乳腺癌、胃癌、结肠癌、膀胱癌、卵巢癌、脑癌和子宫内膜癌相关的多种人类癌症中 ，uPA（纤维蛋白溶酶原激活物，尿激酶）介导的迁移细胞细胞外基质降解对组织重塑、浸润和血管生成至关重要。uPA 促进银屑病和基底细胞癌的发病。 uPA 激活生长因子和金属蛋白酶。
外形
用0.22μm 过滤的 HEPES、NaCl 和 CaCl2 溶液冻干。冻干前一般加入 5-8% 甘露醇或海藻糖作为保护剂。
重悬
打开前离心小瓶。用无菌 PBS（pH 值为 7.4）重建至 50μg/ mL 的浓度。避免涡旋。该溶液可在 2-8°C 下储存长达 1 个月。对于长期存储，建议储存温度为 -20°C。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Increased expression of uPA, uPAR, and PAI-1 in psoriatic skin and in basal cell carcinomas.
Rubina K A, et al.
Archives of Dermatological Research, 309(6), 433-442 (2017)
Enhanced meta-analysis and replication studies identify five new psoriasis susceptibility loci.
Tsoi L C, et al.
Nature Communications, 6, 7001-7001 (2015)
Ajay Kumar et al.
Frontiers in immunology, 12, 807775-807775 (2022-01-04)
Leptospira, a zoonotic pathogen, is known to infect various hosts and can establish persistent infection. This remarkable ability of bacteria is attributed to its potential to modulate (activate or evade) the host immune response by exploiting its surface proteins. We
Jason J Paxman et al.
Nature communications, 10(1), 1967-1967 (2019-05-01)
Autotransporters are the largest family of outer membrane and secreted proteins in Gram-negative bacteria. Most autotransporters are localised to the bacterial surface where they promote colonisation of host epithelial surfaces. Here we present the crystal structure of UpaB, an autotransporter
Up-Regulation of PAI-1 and Down-Regulation of uPA Are Involved in Suppression of Invasiveness and Motility of Hepatocellular Carcinoma Cells by a Natural Compound Berberine.
Wang X
International Journal of Molecular Sciences, 17(4), 577-577 (2016)
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