SRP6271
基质金属蛋白酶-9 人
recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)
别名:
CLG4B, GELB, MANDP2, 基质金属蛋白酶-9
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关于此项目
NACRES:
NA.32
UNSPSC Code:
12352202
Biological source:
human
Recombinant:
expressed in HEK 293 cells
Assay:
≥95% (SDS-PAGE)
Form:
lyophilized powder
Mol wt:
calculated mol wt 50.8 kDa, observed mol wt 55-65 kDa (DTT-reduced. Protein migrates due to different glycosylation. Ala 20 is the predicted N-terminus.)
Impurities:
<1 EU/μg endotoxin (LAL test)
biological source
human
recombinant
expressed in HEK 293 cells
tag
6-His tagged (C-terminus)
assay
≥95% (SDS-PAGE)
form
lyophilized powder
mol wt
calculated mol wt 50.8 kDa, observed mol wt 55-65 kDa (DTT-reduced. Protein migrates due to different glycosylation. Ala 20 is the predicted N-terminus.)
packaging
pkg of 10 and 50 μg
impurities
<1 EU/μg endotoxin (LAL test)
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... MMP-9(4318)
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General description
大多数 MMPs（基质金属蛋白酶）作为无活性的前体蛋白分泌，被细胞外蛋白酶裂解时被激活。基质金属蛋白酶-9 又称 92kDa IV型胶原酶，92 kDa 明胶酶/明胶酶 B (gelatinase/gelatinase B，GELB)，CLG4B，由中性粒细胞、巨噬细胞和编号转化细胞分泌，是结构域结构及其活性调控方面最复杂的家族成员。在结构上，基质金属蛋白酶-9 可分为 5 个不同的结构域：激活后裂解的前结构域、由 3 个连续的纤维连接蛋白 II 类型单位组成的明胶结合结构域、含有锌结合站点的催化结构域、富含脯氨酸的连接区和羧基末端血红素样结构域。
Application
在明胶酶谱法中使用基质金属蛋白酶-9 作为标准品。
Biochem/physiol Actions
基质金属蛋白酶 (matrix metalloproteinase,MMP) 家族的蛋白参与了正常生理过程中细胞外基质的分解，如胚胎发育、繁殖和组织重塑，以及疾病过程，如关节炎和转移。对恒河猴的研究表明基质金属蛋白酶-9 参与 IL-8（白细胞介素-8）诱导的骨髓造血祖细胞的动员，鼠类研究表明其具有肿瘤相关组织重塑中的作用。血小板反应蛋白、椎间盘蛋白，调节基质金属蛋白酶-2 和-9 的有效水平，它们是 ECM（细胞外基质）重塑的关键效应物。这种酶可降解各种底物，包括明胶、IV 型和 V 型胶原以及弹性蛋白。基质金属蛋白酶-9 参与系统性红斑狼疮、类风湿关节炎、多发性硬化等多种自身免疫性疾病，并被视为潜在的治疗靶点。它还与腰椎间盘突出症和干骺端不典型增生有关。
Physical form
在 pH 值为 7.4 的 PBS 中从 0.22 μm 过滤溶液冻干。通常在冻干前加入5-8％甘露醇或海藻糖作为保护剂。
Preparation Note
打开前离心小瓶。用无菌 PBS (pH 值为 7.4) 复溶至 50μg/ mL 的浓度。切勿涡旋。该溶液可在 2-8°C 下储存长达 1 个月。对于长期存储，建议储存温度为 -20°C。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Thrombospondin-1 suppresses spontaneous tumor growth and inhibits activation of matrix metalloproteinase-9 and mobilization of vascular endothelial growth factor.
Rodriguez-Manzaneque JC, et al.
Proceedings of the National Academy of Sciences of the USA, 98, 12485-12490 (2001)
Prevention of interleukin-8-induced mobilization of hematopoietic progenitor cells in rhesus monkeys by inhibitory antibodies against the metalloproteinase gelatinase B (MMP-9).
Pruijt JF, et al.
Proceedings of the National Academy of Sciences of the USA, 96, 10863-10868 (1999)
A functional polymorphism in THBS2 that affects alternative splicing and MMP binding is associated with lumbar-disc herniation.
Hirose Y, et al.
American Journal of Human Genetics, 82, 1122-1129 (2008)
Orlando Maiorani et al.
Scientific reports, 7, 39974-39974 (2017-01-12)
The extracellular matrix glycoprotein EMILIN1 exerts a wide range of functions mainly associated with its gC1q domain. Besides providing functional significance for adhesion and migration, the direct interaction between α4β1 integrin and EMILIN1-gC1q regulates cell proliferation, transducing net anti-proliferative effects.
Involvement of MMP-9 in peribiliary fibrosis and cholangiocarcinogenesis via Rac1-dependent DNA damage in a hamster model.
Prakobwong S, et al.
International Journal of Cancer. Journal International Du Cancer, 127, 2576-2587 (2010)
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