产品名称
ProNGF human, recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC)
biological source
human
recombinant
expressed in E. coli
assay
≥95% (HPLC)
≥95% (SDS-PAGE)
form
lyophilized
mol wt
~25 kDa
packaging
pkg of 10 μg
NCBI accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... NGF(4803)
Application
ProNGF human has been used as a standard in the de-glycosylation experiment for comparative studies by western blotting.
Biochem/physiol Actions
Pro-nerve growth factor (proNGF) participates in fetal neural development. It promotes neuronal survival and differentiation, or apoptosis, by interacting with particular nerve growth factor (NGF) receptors on neurons. Haploinsufficiency of the nerve growth factor β (NGFB) gene results in sensory neuropathy.
The precursor form of the nerve growth factor (proNGF) like its mature form is characterized by the cystine knot motif consisting of three cystine bridges, whereas proneurotrophins and mature neurotrophins elicit opposite biological effects. ProNGF functions preferentially via the complex of pan-neurotrophin receptor p75 (p75NTR) and vps10p domain-containing receptor sortilin inducing neuronal apoptosis and contributing to age- and disease-related neurodegeneration.
General description
The nerve growth factor (NGF) gene produces pro-nerve growth factor (proNGF), a soluble 246 amino acid pro-peptide. NGF belongs to the neurotrophin family. NGF gene is located on human chromosome 1p13.2.
Other Notes
Recombinant human ProNGF produced in E. coli is a non-glycosylated, non-covalently linked homodimer with each polypeptide chain containing 222 amino acids with an extra N-terminal Met and having a molecular mass of 25 kDa. The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Pro-His-Ser.
Physical form
Lyophilized from a 0.2 μm filtered solution of 20 mM PB and 250 mM NaCl, pH 7.2.
Preparation Note
Centrifuge the vial prior to opening.
Dissolve in 1x PBS (It is not recommended to reconstitute to a final concentration less than 100 μg/mL.). This can further be diluted to other aqueous buffers.
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
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Jie Jiang et al.
Journal of cellular and molecular medicine, 24(10), 5901-5910 (2020-04-16)
Pancreatic cancer (PC) is a continuously high lethal disease, and the tumour microenvironment plays a pivotal role during PC progression. Herein, we focus on that the Nerve growth factor (NGF)/Tropomyosin-related kinase A (TrkA), in pancreatic stellate cells-pancreatic cancer cells (PSCs-PC
Christopher W Rowe et al.
BMC endocrine disorders, 19(1), 128-128 (2019-11-30)
Nerves and neurotrophic growth factors are emerging promoters of cancer growth. The precursor for Nerve Growth Factor (proNGF) is overexpressed in thyroid cancer, but its potential role as a clinical biomarker has not been reported. Here we have examined the
Marzia Soligo et al.
Neurochemistry international, 140, 104812-104812 (2020-08-08)
The precursor of Nerve Growth Factor (proNGF) is the predominant form of NGF in the brain, where its tissue levels are increased in neurodegenerative diseases. proNGF exists in two main splicing variants, the long proNGF-A and the short proNGF-B. We
Gregory J Fitzgibbon et al.
Developmental medicine and child neurology, 51(10), 833-837 (2009-02-03)
Pain insensitivity is mediated at the genetic level by the disruption of specific genes associated with neuronal development. Mammalian in vivo and in vitro studies have shown the nerve growth factor (NGF) gene to play an integral role in nerve
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