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重组
expressed in E. coli
方案
≥70% (SDS-PAGE)
表单
buffered aqueous glycerol solution
分子量
~25 kDa
技术
ligand binding assay: suitable
NCBI登记号
运输
dry ice
储存温度
−70°C
基因信息
Escherichia coli IAI39 ... gst> gst
gst(7152360)
一般描述
谷胱甘肽 S-转移酶 (GST) 家族的酶组成了一长串的细胞溶质、线粒体和微粒体蛋白，被用来创建所谓的 GST 基因融合系统。这些蛋白能够与多种底物发生多种反应。在解毒和毒化机制中起角色。GST 标记的大小为 220 个氨基酸，与其他标记（如 myc 或 FLAG-标记）相比相对较大，后者相当小。GST 可用于纯化和检测目的蛋白。在 GST 基因融合系统中，表达介体由编码目的蛋白的基因序列和旁边的 GST 序列组成。诱导表达后，融合蛋白可通过对谷胱甘肽的高亲和力通过亲和层析进行纯化。
应用
无标签 GST 蛋白已在体外肌动蛋白结合试验中使用。它也已被用作TPX2（Xklp2的靶蛋白）的蛋白标签。
外形
以 50 mM Tris-HCl，pH 7.5，150 mM NaCl，10 mM 谷胱甘肽，0.1 mM EDTA，0.25 mM DTT，0.1 mM PMSF，25% 甘油的形式提供。
制备说明
开封后，分装少量，-70℃ 储存。避免重复处理和多次冻融循环。
储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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