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重组
expressed in baculovirus infected Sf9 cells
产品线
PRECISIO® Kinase
方案
≥70% (SDS-PAGE)
表单
buffered aqueous glycerol solution
比活
10.2-13.8 nmol/min·mg
分子量
~69 kDa
NCBI登记号
运输
dry ice
储存温度
−70°C
基因信息
human ... PDK3(5165)
生化/生理作用
PDHK3 or Pyruvate Dehydrogenase Kinase 3 is a member of the PDHK family that phosphorylate and inactivate the Pyruvate Dehydrogenase (PDH). PDH is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. HIF-1 induces PDHK3 expression leading to inhibition of mitochondrial respiration. Forced expression of PDHK3 in cancer cells results in increased lactic acid accumulation and drugs resistance, whereas knocking down PDHK3 inhibits hypoxia-induced cytoplasmic glycolysis and cell survival.
外形
Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
制备说明
after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles
法律信息
PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
储存分类代码
10 - Combustible liquids
WGK
WGK 1
法规信息
常规特殊物品
Chun-Wun Lu et al.
The Journal of biological chemistry, 283(42), 28106-28114 (2008-08-23)
The switch of cellular metabolism from mitochondrial respiration to glycolysis is the hallmark of cancer cells and associated with tumor malignancy. However, the mechanism of this metabolic switch remains largely unknown. Herein, we reported that hypoxia-inducible factor-1 (HIF-1) induced pyruvate
R Gudi et al.
The Journal of biological chemistry, 270(48), 28989-28994 (1995-12-01)
Recent evidence from this laboratory indicates that at least two isoenzymic forms of pyruvate dehydrogenase kinase (PDK1 and PDK2) may be involved in the regulation of enzymatic activity of mammalian pyruvate dehydrogenase complex by phosphorylation (Popov, K.M., Kedishvili, N.Y., Zhao
商品
本页面介绍了一篇有关瓦博格效应的文章，以及其如何能够在正常氧气水平下，增强肿瘤细胞中葡萄糖向乳酸的转化。Otto Heinrich Warburg在1924年证明，癌细胞显示出对糖酵解的依赖性增加，以满足他们的能量需求，无论是否有充足的氧气存在。
Warburg effect enhances glucose to lactate conversion in tumor cells, regardless of oxygen levels; impacting cancer metabolism since 1924.
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