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生物来源
human
重组
expressed in baculovirus infected Sf9 cells
产品线
PRECISIO® Kinase
检测方案
≥70% (SDS-PAGE)
形式
buffered aqueous glycerol solution
比活
63-85 nmol/min·mg
分子量
~95 kDa
NCBI登记号
应用
cell analysis
运输
dry ice
储存温度
−70°C
基因信息
human ... PAK1(5058)
一般描述
p21 (RAC1) activated kinase 1 (PAK1) is a member of the p21-activated kinases (PAKs) which have been implicated in the regulation of cell morphology, motility and transformation. PAK1 belongs to the Group I PAKs which also includes PAK2 and PAK3. The gene encoding this protein is localized on human chromosome 11q13.
生化/生理作用
p21-activated kinases (PAKs) are serine/threonine kinases that are activated by and are effectors of small GTPases, cell division control protein 42 (CDC42) and Ras-related C3 botulinum toxin substrate (RAC). PAK1 is a key regulator of the actin cytoskeleton, adhesion and cell motility. Inactive dimeric PAK1 is mainly cytosolic and interaction with the activators Cdc42-GTP and Rac1-GTP stimulates the kinase at the sites of cellular protrusions forming adhesions to the extracellular matrix. The protein has a role in numerous cellular processes and has been shown to be expressed in squamous non-small cell lung carcinomas.
外形
Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
制备说明
after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles
法律信息
PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 1
法规信息
常规特殊物品
Dynamics of the Rho-family small GTPases in actin regulation and motility.
Spiering D
Cell adhesion & migration, 5(2), 170-180 (2011)
Essential role of CIB1 in regulating PAK1 activation and cell migration.
Leisner TM
The Journal of Cell Biology, 170(3), 465-476 (2005)
Targeting p21-activated kinase 1 (PAK1) to induce apoptosis of tumor cells.
Ong CC
Proceedings of the National Academy of Sciences of the USA, 108(17), 7177-7182 (2011)
M C Parrini et al.
Biochemical Society transactions, 33(Pt 4), 646-648 (2005-07-27)
Pak1 (p21-activated kinase 1) is a key regulator of the actin cytoskeleton, adhesion and cell motility. Such biological roles require a tight spatial and kinetic control of its localization and activity. We summarize here the current knowledge on Pak1 dynamics
Zahara M Jaffer et al.
The international journal of biochemistry & cell biology, 34(7), 713-717 (2002-04-16)
The p21-activated kinases (Paks) are serine/threonine protein kinases that bind to and, in some cases, are stimulated by activated forms of the small GTPases, Cdc42 and Rac. With the recent discovery of several novel isoforms, Paks are now categorized into
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