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Merck
CN

SRP5150

Sigma-Aldrich

Vimentin, His tagged human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE), buffered aqueous glycerol solution

别名:

FLJ36605, VIM

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About This Item

UNSPSC代码:
12352202
NACRES:
NA.32

生物来源

human

重组

expressed in E. coli

方案

≥90% (SDS-PAGE)

表单

buffered aqueous glycerol solution

分子量

~60 kDa

NCBI登记号

应用

cell analysis

运输

dry ice

储存温度

−70°C

基因信息

human ... VIM(7431)

一般描述

Vimentin belongs to the intermediate filament family of proteins. It is a key structural component of eukaryotic cells and is associated with lymphocytes and neutrophils. Vimentin exists as a tripartite domain structure. It is expressed in mesenchymal cells. The vimentin gene is mapped to human chromosome 10p13.

应用

Vimentin, His tagged human has been used in the glass surface immobilization in anti-vimentin binding in force measurement by atomic force microscopy (AFM).

生化/生理作用

Vimentin (VIM) stabilizes cytoskeletal interactions and is crucial for the placement of organelles in the cytosol. It modulates apoptosis in lymphocytes and is involved in the pathogenesis of inflammatory diseases VIM may serve as a potential marker for sepsis diagnosis. It is also essential for lens integrity maintenance.
Vimentin is a member of intermediate filament family of proteins and is an important structural feature of eukaryotic cells. Along with microtubules and actin microfilaments, Vimentin make up the cytoskeleton component of cells. Studies have shown that Vimentin is attached to the nucleus, endoplasmic reticulum and mitochondria, either laterally or terminally. Vimentin plays a significant role in supporting and anchoring the position of the organelles in the cytosol. Thus, Vimentin plays a key role in maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions

外形

Supplied in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.

制备说明

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

象形图

Health hazardExclamation mark

警示用语:

Danger

危险声明

危险分类

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

储存分类代码

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 1

法规信息

常规特殊物品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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T Katsumoto et al.
Biology of the cell, 68(2), 139-146 (1990-01-01)
The three-dimensional arrangement of vimentin intermediate filaments (IF) was studied in 3Y1, rat fibroblastic cell line, to elucidate its biological role in the cell. While actin filaments were observed exclusively in the superficial part of the cell, vimentin IF were
Vera L Costa et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 16(23), 5842-5851 (2010-10-27)
To identify a panel of epigenetic biomarkers for accurate bladder cancer (BlCa) detection in urine sediments. Gene expression microarray analysis of BlCa cell lines treated with 5-aza-2'-deoxycytidine and trichostatin A as well as 26 tissue samples was used to identify
Frida Danielsson et al.
Cells, 7(10) (2018-09-27)
Vimentin is a protein that has been linked to a large variety of pathophysiological conditions, including cataracts, Crohn's disease, rheumatoid arthritis, HIV and cancer. Vimentin has also been shown to regulate a wide spectrum of basic cellular functions. In cells
Longxiang Su et al.
Scientific reports, 9(1), 5747-5747 (2019-04-07)
New diagnostic biomarkers or therapeutic targets for sepsis have substantial significance for critical care medicine. In this study, 192 differentially expressed proteins were selected through iTRAQ. Based on cluster analysis of protein expression dynamics and protein-protein interactions, hemopexin, vimentin, and
Intermediate filaments: structure, dynamics, function, and disease.
E Fuchs et al.
Annual review of biochemistry, 63, 345-382 (1994-01-01)

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