SRP5127
RhoA，His 标记 人
recombinant, expressed in E. coli, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
别名:
ARH12, ARHA, RHO12, RHOH12
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关于此项目
NACRES:
NA.32
UNSPSC Code:
12352200
Form:
buffered aqueous glycerol solution
Assay:
≥70% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in E. coli
Mol wt:
~23 kDa
产品名称
RhoA，His 标记 人, recombinant, expressed in E. coli, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
biological source
human
recombinant
expressed in E. coli
assay
≥70% (SDS-PAGE)
form
buffered aqueous glycerol solution
mol wt
~23 kDa
NCBI accession no.
application(s)
cell analysis
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... RHOA(387)
Application
RhoA, His tagged human has been used for in vitro kinase assay.
Biochem/physiol Actions
Ras homolog family member A (RhoA) interacts with protein kinases and serves as a target of activated GTPase. RhoA plays an important role in the initial events of cell protrusion. Rho mediates corneal epithelial migration in response to external stimuli by regulating the organization of the actin cytoskeleton. The inhibition of RhoA signaling is required for both vaccinia morphogenesis and virus-induced cell motility. Vaccinia inhibits RhoA signaling using the viral protein F11 and this can enhance the spread of infection. The protein has a role in basal body docking and planar polarization.
General description
Ras homolog family member A (RhoA) is a small GTPase which is part of the Rho gene family. The gene encoding it is localized on human chromosome 3p21.31.
Physical form
Supplied in 50mM MOPS, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
Preparation Note
after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
存储类别
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Activation of RHOA gene transcription in epithelial tumors may be caused by gene amplification and/or demethylation of its promotor region
Molecular Biology (2006)
Connective Tissue Growth Factor in Regulation of RhoA Mediated Cytoskeletal Tension Associated Osteogenesis of Mouse Adipose-Derived Stromal Cells
Yue Xu
PLoS ONE (2010)
Coordination of Rho GTPase activities during cell protrusion.
Machacek M
Nature (2009)
Jin-Hee Kim et al.
The pharmacogenomics journal, 20(4), 601-612 (2020-02-06)
Previously, we identified Ras homologous A (RHOA) as a major signaling hub in gastric cancer (GC), the third most common cause of cancer death in the world, prompting us to rationally design an efficacious inhibitor of this oncogenic GTPase. Here
Phosphodiesterase 5 Inhibition Limits Doxorubicin-induced Heart Failure by Attenuating Protein Kinase G Ia Oxidation*
Oleksandra P
The Journal of Biological Chemistry (2016)
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