产品名称
G-CSF from mouse, Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)
biological source
mouse
recombinant
expressed in E. coli
assay
≥98% (HPLC)
≥98% (SDS-PAGE)
form
lyophilized
potency
≤0.05 ng/mL
mol wt
19.0 kDa
packaging
pkg of 10 μg
impurities
endotoxin, tested
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
mouse ... CSF3(12985)
Biochem/physiol Actions
Colony stimulating factor 3 (CSF-3) or granulocyte colony stimulating factor (G-CSF) stimulates the development of committed progenitor cells to neutrophils and enhances the functional activities of the mature end-cell. It also has a role in macrophage generation and may be involved in immune modulation. G-CSF is being used clinically to facilitate hematopoietic recovery after bone marrow transplantation.
General description
Colony stimulating factor 3 (CSF-3) or granulocyte colony stimulating factor (G-CSF) is a hematopoietic growth factor. It is produced in response to specific stimulation by a variety of cells including macrophages, fibroblasts, endothelial cells and bone marrow stroma. Human and murine G-CSF is cross-species reactive. Recombinant murine G-CSF is a 19.0kDa protein consisting of 179 amino acid residues.
Physical form
Lyophilized from 10 mM Sodium Citrate, pH 4.0.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
The importance of the granulocyte-colony stimulating factor in oncology.
Cetean S
Clujul Medical (1957), 88(4), 468-472 (2015)
H S Lu et al.
Archives of biochemistry and biophysics, 268(1), 81-92 (1989-01-01)
Molecular characteristics and secondary structures of recombinant methionyl human granulocyte colony stimulating factor produced by genetically engineered Escherichia coli are described. Limited radiolabeling of the protein with tritiated iodoacetate and determination of the labeled residue revealed that this recombinant protein
Protective role of G-CSF in dextran sulfate sodium-induced acute colitis through generating gut-homing macrophages.
Meshkibaf S
Cytokine, 78, 69-78 (2016)
Gerald Wirnsberger et al.
Nature genetics, 46(9), 1028-1033 (2014-08-19)
Neutrophils are key innate immune effector cells that are essential to fighting bacterial and fungal pathogens. Here we report that mice carrying a hematopoietic lineage-specific deletion of Jagn1 (encoding Jagunal homolog 1) cannot mount an efficient neutrophil-dependent immune response to
Yaron Vagima et al.
PLoS pathogens, 11(5), e1004893-e1004893 (2015-05-15)
Pneumonic plague is a fatal disease caused by Yersinia pestis that is associated with a delayed immune response in the lungs. Because neutrophils are the first immune cells recruited to sites of infection, we investigated the mechanisms responsible for their
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