生物来源
mouse
重组
expressed in E. coli
检测方案
≥98% (HPLC)
≥98% (SDS-PAGE)
形式
lyophilized
效能
0.08-0.12 μg/mL
分子量
27.0 kDa
包装
pkg of 50 μg
杂质
<0.1 EU/μg endotoxin, tested
颜色
white to off-white
UniProt登记号
运输
wet ice
储存温度
−20°C
基因信息
mouse ... GDF5(14563)
一般描述
Mutations in the GDF-5 gene have been implicated in Grebe Syndrome, which is characterized by short stature, extra digits, short and deformed extremities, and in Hunter-Thompson type dwarfism. The mature and functional form of GDF-5 is a homodimer of two 120 amino-acid polypeptide chain (monomers) linked by a single disulfide bond. Each GDF-5 monomer is expressed as the C-terminal part of a precursor polypeptide, which also contains a 27 amino-acid signal peptide and a 348 amino-acid propeptide. This precursor undergoes intracellular dimerization, and upon secretion it is processed by a furin-type protease.
生化/生理作用
GDF-5 is expressed in long bones during embryonic development and postnatally in articular cartilage. Recombinant murine GDF-5 is a 27.0 kDa homodimeric disulfide-linked protein consisting of two 120 amino acids.
外形
Lyophilized with no additives.
重悬
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
监管及禁止进口产品
Eiichi Hinoi et al.
Biochemical and biophysical research communications, 450(1), 255-260 (2014-06-20)
We have previously demonstrated promotion by growth differentiation factor-5 (GDF5) of brown adipogenesis for systemic energy expenditure through a mechanism relevant to activating the bone morphological protein (BMP) receptor/mothers against decapentaplegic homolog (Smad)/peroxisome proliferator-activated receptor gamma co-activator 1α (PGC-1α) pathway.
Cartilage-derived morphogenetic proteins. New members of the transforming growth factor-beta superfamily predominantly expressed in long bones during human embryonic development.
Chang, C.H. et al.
The Journal of Biological Chemistry, 45, 28227-28234 (1994)
Zhou Pei et al.
Archives of biochemistry and biophysics, 560, 27-35 (2014-08-01)
Adipocyte differentiation is key to determining the number of adipocytes during the development of obesity. Recent studies have shown that growth differentiation factor-5 (GDF5) promotes brown adipogenesis, however its role in white adipogenesis is still uncertain. The aim of the
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