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安全信息

SRP3149

Sigma-Aldrich

sCD14 human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture

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别名:
soluble CD14
UNSPSC代码:
12352202
NACRES:
NA.32

生物来源

human

重组

expressed in HEK 293 cells

检测方案

≥95% (HPLC)
≥95% (SDS-PAGE)

形式

lyophilized

分子量

41.6 kDa

包装

pkg of 50 μg

技术

cell culture | mammalian: suitable

杂质

<0.1 EU/μg endotoxin, tested

颜色

white

UniProt登记号

运输

wet ice

储存温度

−20°C

基因信息

human ... CD14(929)

序列

TTPEPCELDD EDFRCVCNFS EPQPDWSEAF QCVSAVEVEI HAGGLNLEPF LKRVDADADP RQYADTVKAL RVRRLTVGAA QVPAQLLVGA LRVLAYSRLK ELTLEDLKIT GTMPPLPLEA TGLALSSLRL RNVSWATGRS WLAELQQWLK PGLKVLSIAQ AHSPAFSCEQ VRAFPALTSL DLSDNPGLGE RGLMAALCPH KFPAIQNLAL RNTGMETPTG VCAALAAAGV QPHSLDLSHN SLRATVNPSA PRCMWSSALN SLNLSFAGLE QVPKGLPAKL RVLDLSCNRL NRAPQPDELP EVDNLTLDGN PFLVPGTALP HEGSMNSGVV P

外形

Lyophilized from 10 mM Sodium Phosphate, pH 7.5.

重悬

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

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Binding of CD14 to Mycoplasma genitalium-derived lipid-associated membrane proteins upregulates TNF-a.
He J
Inflammation, 37(2), 322-330 (2014)
Richard L Kitchens et al.
Journal of endotoxin research, 11(4), 225-229 (2005-09-24)
LPS binding protein (LBP) and CD14 play key roles in promoting innate immunity to Gram-negative bacteria by transferring LPS to the signaling receptor complex, MD-2/Toll-like receptor 4 (TLR4). LBP and soluble CD14 (sCD14) can also inhibit responses to LPS by
Time to abandon dogma: CD14 is expressed by non-myeloid lineage cells.
Jersmann HP
Immunology and Cell Biology, 83(5), 462-467 (2005)
Proteolysis of monocyte CD14 by human leukocyte elastase inhibits lipopolysaccharide-mediated cell activation.
Le-Barillec K
The Journal of Clinical Investigation, 103(7), 1039-1046 (1999)
CD14 protein acts as an adaptor molecule for the immune recognition of Salmonella curli fibers.
Rapsinski GJ
The Journal of Biological Chemistry, 288(20), 14178-14188 (2013)

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