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主要文件

安全信息

SRP3149

Sigma-Aldrich

sCD14 human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE), ≥95% (HPLC), suitable for cell culture

别名:

soluble CD14

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About This Item

UNSPSC代码:
12352202
NACRES:
NA.32

生物来源

human

重组

expressed in HEK 293 cells

方案

≥95% (HPLC)
≥95% (SDS-PAGE)

表单

lyophilized

分子量

41.6 kDa

包装

pkg of 50 μg

技术

cell culture | mammalian: suitable

杂质

<0.1 EU/μg endotoxin, tested

颜色

white

UniProt登记号

运输

wet ice

储存温度

−20°C

基因信息

human ... CD14(929)

序列

TTPEPCELDD EDFRCVCNFS EPQPDWSEAF QCVSAVEVEI HAGGLNLEPF LKRVDADADP RQYADTVKAL RVRRLTVGAA QVPAQLLVGA LRVLAYSRLK ELTLEDLKIT GTMPPLPLEA TGLALSSLRL RNVSWATGRS WLAELQQWLK PGLKVLSIAQ AHSPAFSCEQ VRAFPALTSL DLSDNPGLGE RGLMAALCPH KFPAIQNLAL RNTGMETPTG VCAALAAAGV QPHSLDLSHN SLRATVNPSA PRCMWSSALN SLNLSFAGLE QVPKGLPAKL RVLDLSCNRL NRAPQPDELP EVDNLTLDGN PFLVPGTALP HEGSMNSGVV P

外形

Lyophilized from 10 mM Sodium Phosphate, pH 7.5.

重悬

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

从最新的版本中选择一种:

分析证书(COA)

Lot/Batch Number

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Richard L Kitchens et al.
Journal of endotoxin research, 11(4), 225-229 (2005-09-24)
LPS binding protein (LBP) and CD14 play key roles in promoting innate immunity to Gram-negative bacteria by transferring LPS to the signaling receptor complex, MD-2/Toll-like receptor 4 (TLR4). LBP and soluble CD14 (sCD14) can also inhibit responses to LPS by
Binding of CD14 to Mycoplasma genitalium-derived lipid-associated membrane proteins upregulates TNF-a.
He J
Inflammation, 37(2), 322-330 (2014)
CD14 protein acts as an adaptor molecule for the immune recognition of Salmonella curli fibers.
Rapsinski GJ
The Journal of Biological Chemistry, 288(20), 14178-14188 (2013)
Modulation of the lipopolysaccharide receptor complex (CD14, TLR4, MD-2) and toll-like receptor 2 in systemic inflammatory response syndrome-positive patients with and without infection: relationship to tolerance.
Calvano JE
Shock, 20(5), 415-419 (2003)
Proteolysis of monocyte CD14 by human leukocyte elastase inhibits lipopolysaccharide-mediated cell activation.
Le-Barillec K
The Journal of Clinical Investigation, 103(7), 1039-1046 (1999)

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