生物来源
human
重组
expressed in E. coli
检测方案
≥98% (HPLC)
≥98% (SDS-PAGE)
形式
lyophilized
分子量
22.4 kDa
包装
pkg of 10 μg
技术
cell culture | mammalian: suitable
杂质
<0.1 EU/μg endotoxin, tested
颜色
white to off-white
UniProt登记号
运输
wet ice
储存温度
−20°C
基因信息
human ... CLCF1(23529)
一般描述
Novel neurotrophin-1/B-cell stimulating factor-3 (NNT-1/BSF-3), also known as cardiotrophin-like cytokine factor 1 (CLCF1), is encoded by the gene mapped to human chromosome 11q13.2. The encoded protein is a new member of the gp130 cytokine family. Recombinant human NNT-1/BCSF-3 is a 22.4 kDa protein containing 199 amino acid residues.
生化/生理作用
Novel neurotrophin-1/B-cell stimulating factor-3 (NNT-1/BSF-3) has neurotrophic and B cell stimulatory effects.In addition, it is also implicated in neuroimmunoendocrine modulation of corticotroph function. BSF-3 helps in the embryonic development and differentiation of motor, sensory and autonomic neurons. It also facilitates the bone formation during development and in the ongoing remodeling of endochondral bone. The encoded protein binds and activates glycoprotein 130 (gp130) and leukemia inhibitory factor receptor member b (LIFR-b). This ligand and receptor interaction further induces downstream signaling events and activates Janus kinase 1 (JAK1)-signal transducer and activator of transcription protein 3 (STAT3) signaling pathway. In vitro, it supports the survival of chick embryo motor and sympathetic neurons. In mice, NNT-1/BCSF-3 induces serum amyloid A, causes body weight loss and B cell hyperplasia associated with increased in serum IgG and IgM.
序列
MLNRTGDPGP GPSIQKTYDL TRYLEHQLRS LAGTYLNYLG PPFNEPDFNP PRLGAETLPR ATVDLEVWRS LNDKLRLTQN YEAYSHLLCY LRGLNRQAAT AELRRSLAHF CTSLQGLLGS IAGVMAALGY PLPQPLPGTE PTWTPGPAHS DFLQKMDDFW LLKELQTWLW RSAKDFNRLK KKMQPPAAAV TLHLGAHGF
外形
Lyophilized with no additives.
重悬
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
Cold-induced sweating syndrome including Crisponi syndrome.
GeneReviews(?) (2011)
Novel neurotrophin-1/B cell-stimulating factor-3 (NNT-1/BSF-3) / cardiotrophin-like cytokine (CLC)?a novel gp130 cytokine with pleiotropic functions
Cytokine & Growth Factor Reviews, 15(5), 325-336 (2004)
Proceedings of the National Academy of Sciences of the United States of America, 96(20), 11458-11463 (1999-09-29)
We have identified a cytokine of the IL-6 family and named it novel neurotrophin-1/B cell-stimulating factor-3 (NNT-1/BSF-3). NNT-1/BSF-3 cDNA was cloned from activated Jurkat human T cell lymphoma cells. Its sequence predicts a 225-aa protein with a 27-aa signal peptide
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