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一般描述
Recombinant human Midkine is a 13.4 kDa protein containing 123 amino acid residues including five intra-molecular disulfide bonds.
应用
Midkine human has been used in cell viability assay and cell proliferation assay to determine its association with EMT (epithelial-mesenchymal transition) and gemcitabine resistance in biliary tract cancer.
生化/生理作用
Midkine (MK) and its functionally-related protein Pleiotrophin are heparin-binding neurotrophic factors that signal through the same receptor, known as anaplastic lymphoma kinase (ALK). MK (midkine) plays an important regulatory role in epithelial-mesenchymal interactions during fetal development and in postnatal lung development. MK chemoattracts embryonic neurons, neutrophils and macrophages. Signaling through the ALK (anaplastic lymphoma kinase) receptor MK exerts angiogenic, growth and survival activities during tumorigenesis. MK is expressed predominantly during midgestation in the brain and several other organs and is down-regulated at birth.
序列
VAKKKDKVKK GGPGSECAEW AWGPCTPSSK DCGVGFREGT CGAQTQRIRC RVPCNWKKEF GADCKYKFEN WGACDGGTGT KVRQGTLKKA RYNAQCQETI RVTKPCTPKT KAKAKAKKGK GKD
外形
Lyophilized from 0.4x PBS, pH 7.4.
重悬
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
监管及禁止进口产品
Midkine binds to anaplastic lymphoma kinase (ALK) and acts as a growth factor for different cell types.
Stoica G E, et al.
The Journal of Biological Chemistry, 277(39), 35990-35998 (2002)
The growth factor midkine is modulated by both glucocorticoid and retinoid in fetal lung development.
Kaplan F, et al.
American Journal of Respiratory Cell and Molecular Biology, 28(1), 33-41 (2003)
Midkine regulates pleiotrophin organ-specific gene expression: evidence for transcriptional regulation and functional redundancy within the pleiotrophin/midkine developmental gene family.
Herradon G, et al.
Biochemical and Biophysical Research Communications, 333(3), 714-721 (2005)
Hui-Lian Huang et al.
International journal of clinical and experimental pathology, 8(5), 5387-5394 (2015-07-21)
To investigate the inhibitory effect of midkine-binding peptides on human umbilical vein endothelial cells (HUVECs) proliferation and angiogenesis of xenograft tumor. The midkine-binding peptides were panned by Ph.D.-7(™) Phage Display Peptide Library Kit, and the specific binding activities of positive
Effect of midkine on gemcitabine resistance in biliary tract cancer.
Lu Y, et al.
International Journal of Molecular Medicine, 41(4), 2003-2011 (2018)
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