生物来源
human
重组
expressed in E. coli
检测方案
≥85% (SDS-PAGE)
形式
frozen liquid
分子量
~33.6 kDa
包装
pkg of 10 μg
储存条件
avoid repeated freeze/thaw cycles
浓度
300 μg/mL
颜色
clear colorless
NCBI登记号
UniProt登记号
运输
dry ice
储存温度
−70°C
基因信息
human ... POLR2L(5441)
生化/生理作用
The RNA polymerase subunit RPB10 displays a high level of conservation across archaea and eukarya and is required for cell viability in yeast. It is a zinc-binding protein with an atypical CX2CXnCC metal binding motif. RPB10 participates in protein-protein interactions with additional components of the polymerase holoenzyme. Experimentally, the binding of RPB10 to an RPB3-RPB11 (or RPAC40-RPAC19 in RNAPI/III) heterodimer is well characterized and persists in both eukaryal and archaeal RNAPs. Binding of RPB10 to this a2-like heterodimer through zinc-mediated hemi-coordination suggests an early role in holoenzyme assembly since the formation of the α2-complex is the first step in the assembly of the prokaryotic RNAPs.
外形
Clear and colorless frozen liquid solution
制备说明
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.
储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
C D Mackereth et al.
Proceedings of the National Academy of Sciences of the United States of America, 97(12), 6316-6321 (2000-06-07)
The RNA polymerase subunit RPB10 displays a high level of conservation across archaea and eukarya and is required for cell viability in yeast. Structure determination of this RNA polymerase subunit from Methanobacterium thermoautotrophicum reveals a topology, which we term a
N A Woychik et al.
Trends in biochemical sciences, 15(9), 347-351 (1990-09-01)
RNA polymerase II is the core of the complex apparatus that is responsible for the regulated synthesis of mRNA. A comprehensive knowledge of RNA polymerase II is essential to our understanding of the molecular mechanisms through which a variety of
N A Woychik et al.
Genes & development, 4(3), 313-323 (1990-03-01)
RNA polymerases I, II, and III share three subunits that are immunologically and biochemically indistinguishable. The Saccharomyces cerevisiae genes that encode these subunits (RPB5, RPB6, and RPB8) were isolated and sequenced, and their transcriptional start sites were deduced. RPB5 encodes
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