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![GAL4 [(1-147) - AH] from Saccharomyces cerevisiae recombinant, expressed in E. coli, ≥80% (SDS-PAGE)](/deepweb/assets/sigmaaldrich/product/images/174/140/27ccc70c-c146-4db8-888e-496728638bd2/640/27ccc70c-c146-4db8-888e-496728638bd2.jpg)
生物来源
Saccharomyces cerevisiae
canine
重组
expressed in E. coli
方案
≥70% (SDS-PAGE)
表单
frozen liquid
分子量
~33 kDa
包装
pkg of 4 μg
储存条件
avoid repeated freeze/thaw cycles
浓度
100 μg/mL
技术
electrophoretic mobility shift assay: suitable
颜色
colorless to clear
NCBI登记号
UniProt登记号
运输
dry ice
储存温度
−70°C
基因信息
Saccharomyces cerevisiae ... GAL4(855828)
生化/生理作用
The GAL4 protein of yeast activates the transcription of several genes involved in galactose metabolism. This event requires that GAL4 bind to upstream activation sites with the consensus sequence 5`-CGGN5(T/A)N5CCG-3` . A fragment of the GAL4 protein, comprising amino acids 1-147, binds DNA but fails to activate transcription. The adenovirus E1A protein stimulates transcription of a wide variety of viral and cellular genes. In addition to its trans-activating functions, E1A is also able to modulate progression through the cell cycle, to immortalize primary cells in culture and to induce cellular transformation. The E1A protein binds to the TATA-binding protein, TBP, TAFII55 and TAFII110. Its activating domain has been shown to interact with E2F, ATF-2 and YY1.
外形
Clear and colorless frozen liquid solution
制备说明
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.
储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
Pre-early adenovirus 5 gene product regulates synthesis of early viral messenger RNAs.
A J Berk et al.
Cell, 17(4), 935-944 (1979-08-01)
L Keegan et al.
Science (New York, N.Y.), 231(4739), 699-704 (1986-02-14)
The yeast GAL4 protein (881 amino acids) binds to specific DNA sites upstream of target genes and activates transcription. Derivatives of this protein bearing as few as 74 amino terminal residues bind to these sites but fail to activate transcription.
T Kodadek
Cellular & molecular biology research, 39(4), 355-360 (1993-01-01)
The GAL4 protein of yeast activates the transcription of several genes involved in galactose metabolism. This event requires that GAL4 bind to upstream activation sites with the consensus sequence 5'-CGGN5(T/A)N5CCG-3'. We review the general requirements that must be met for
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