生物来源
human
重组
expressed in E. coli
方案
≥80% (SDS-PAGE)
表单
frozen liquid
分子量
~84 kDa
包装
pkg of 10 μg
储存条件
avoid repeated freeze/thaw cycles
浓度
400 μg/mL
颜色
clear colorless
UniProt登记号
运输
dry ice
储存温度
−70°C
基因信息
human ... GTF2F1(2962) , GTF2F2(2963)
生化/生理作用
The transcription factor IIF (TFIIF) is composed of 58 kDa (RAP74) and 26 kDa (RAP34) subunits as a heterodimer, and was first identified through the ability to interact with immobilized RNA polymerase II. The Rap74 subunit of TFIIF can be phosphorylated by TAF250 both in vivo and in vitro. The RAP30 subunit of TFIIF contains two distinct regions with sequence similarity to E. coli s factors and can deliver RNA polymerase II to the promoter to support transcription initiation in the absence of RAP74.
外形
Clear and colorless frozen liquid solution
制备说明
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.
储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
S McCracken et al.
Science (New York, N.Y.), 253(5022), 900-902 (1991-08-23)
RAP30/74 is a heteromeric general transcription initiation factor that binds to mammalian RNA polymerase II. The RAP30 subunit contains a region that is similar in amino acid sequence to the RNA polymerase-binding domain of the Escherichia coli transcription initiation factor
M Sopta et al.
Nature, 341(6241), 410-414 (1989-10-05)
RAP30/74 is a heteromeric general transcription initiation factor which binds to RNA polymerase II. Here we report that preparations of RAP30/74 contain an ATP-dependent DNA helicase whose probable function is to melt the DNA at transcriptional start sites. The sequence
M Sopta et al.
The Journal of biological chemistry, 260(18), 10353-10360 (1985-08-25)
We have used affinity chromatography on columns containing immobilized calf thymus RNA polymerase II to isolate three phosphoproteins (RAP72, RAP38, and RAP30) that bind directly to RNA polymerase II. All could be isolated from cell nuclei, and all three could
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