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生物来源
human
重组
expressed in E. coli
检测方案
≥90% (SDS-PAGE)
形式
aqueous solution
分子量
113.8 kDa
包装
pkg of 100 μg
运输
dry ice
储存温度
−70°C
基因信息
human ... HIST1H2BG(8339) , HIST2H2AC(8338) , HIST3H3(8290) , HIST4H4(121504)
一般描述
Human recombinant histone octamer consisting of 2 molecules each of histones H2A (GenBank Accession No. NM_033445) amino acids 2-130(end) with a N-terminal His-tag, H2B (GenBank Accession No. NM_003528) amino acids 2-126(end) with a N-terminal His-tag, H3 (GenBank Accession No. NM_003532) amino acids 2-137(end) with a N-terminal His-tag, and H4 (GenBank Accession No. NM_003548) amino acids 2-103(end) with a N-terminal His-tag, expressed in an E. coli expression system.
生化/生理作用
The nuclear DNA in eukaryotes is found to be associated with histones to form a compact complex called nucleosome. Histones neutralize the electrostatic nature of DNA and function as scaffolding proteins. Each core nucleosome contains two copies each of the core histones H2A, H2B, H3, and H4 to form an octameric complex. This octameric complex contains a central (H3-H4)2 tetramer flanked on both sides with H2A-H2B dimers. The octamer complex function in various stages of chromosome function, chromatin assembly and nucleosome formation. The histone dimer-tetramer interactions are also important in RNA transcription.
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Vishal V Raut et al.
Annals of botany, 108(7), 1235-1246 (2011-09-08)
In eukaryotes, chromatin remodelling complexes are shown to be responsible for nucleosome mobility, leading to increased accessibility of DNA for DNA binding proteins. Although the existence of such complexes in plants has been surmised mainly at the genetic level from
The nucleosomal core histone octamer at 3.1 A resolution: a tripartite protein assembly and a left-handed superhelix.
Arents G
Proceedings of the National Academy of Sciences of the USA, 88, 10148-10152 (1991)
Histone octamer function in vivo: mutations in the dimer-tetramer interfaces disrupt both gene activation and repression.
Santisteban MS
The Embo Journal, 16, 2493-2506 (1997)
Kyle M Miller et al.
Biochemical Society transactions, 40(2), 370-376 (2012-03-23)
Inherited or acquired defects in detecting, signalling or repairing DNA damage are associated with various human pathologies, including immunodeficiencies, neurodegenerative diseases and various forms of cancer. Nuclear DNA is packaged into chromatin and therefore the true in vivo substrate of
Shrika G Harjivan et al.
Molecules (Basel, Switzerland), 26(5) (2021-04-04)
Nevirapine (NVP), a non-nucleoside reverse transcriptase inhibitor widely used in combined antiretroviral therapy and to prevent mother-to-child transmission of the human immunodeficiency virus type 1, is associated with several adverse side effects. Using 12-mesyloxy-nevirapine, a model electrophile of the reactive
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