生物来源
human
重组
expressed in baculovirus infected Sf9 cells
检测方案
≥40% (SDS-PAGE)
形式
aqueous solution
分子量
60 kDa
包装
pkg of 10 μg
储存条件
avoid repeated freeze/thaw cycles
浓度
0.4 mg/mL
NCBI登记号
运输
dry ice
储存温度
−70°C
基因信息
human ... CERK(64781)
一般描述
Ceramide kinase (CERK) is a 537-amino acid protein. It has a catalytic region, a putative amino-myristoylation site in its amino terminal region and a pleckstrin homology domain. The gene encoding CERK is localized on human chromosome 22q13.31.
Human ceramide kinase (CERK) (GenBank Accession No. NM_022766), 1-537 (end) with C-terminal Flag-tag, MW= 60 kDa, expressed in Baculovirus expression system.
应用
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
生化/生理作用
Ceramide kinase (CERK) is an enzyme involved in sphingolipid metabolism and in the synthesis of ceramide-1-phosphate. It associates with calmodulin and at the time of Fc receptor-mediated phagocytosis, it aggregates in lipid rafts. CERK may have a role in calcium signaling. The protein functions in the phagocytosis of polymorphonuclear leukocytes and degranulation of mast cells.
单位定义
One unit is defined as the amount of enzyme required to produce 1 pmole of ceramide-1-phosphate/min at 37°C.
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
Ceramide kinase: a novel lipid kinase molecular cloning and functional characterization
Sugiura M, et al.
The Journal of Biological Chemistry (2002)
Tack-Joong Kim et al.
Biochemical and biophysical research communications, 342(2), 611-617 (2006-02-21)
Ceramide kinase (CERK) converts ceramide to ceramide-1-phosphate (C1P), which has recently emerged as a new bioactive molecule capable of regulating diverse cellular functions. The N-terminus of the CERK protein encompasses a sequence motif known as a pleckstrin homology (PH) domain.
A conserved cysteine motif essential for ceramide kinase function
Lidome E, et al.
Biochimie, 90(10), 1560-1565 (2008)
Francesca Bini et al.
Neuropharmacology, 63(4), 524-537 (2012-05-15)
1α,25-Dihydroxyvitamin D3 (1,25(OH)₂D₃), a crucial regulator of calcium/phosphorus homeostasis, has important physiological effects on growth and differentiation in a variety of malignant and non-malignant cells. Synthetic structural hormone analogues, with lower hypercalcemic side effects, are currently under clinical investigation. Sphingolipids
Vania Hinkovska-Galcheva et al.
Journal of lipid research, 49(3), 531-542 (2007-12-25)
Ceramide-1-phosphate (C1P) is a novel bioactive sphingolipid formed by the phosphorylation of ceramide catalyzed by ceramide kinase (CERK). In this study, we evaluated the mechanism by which increased C1P during phagocytosis enhances phagocytosis and phagolysosome formation in COS-1 cells expressing
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