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生物来源
human
重组
expressed in baculovirus infected Sf9 cells
检测方案
≥70% (SDS-PAGE)
形式
aqueous solution
分子量
83 kDa
包装
pkg of 100 μg
NCBI登记号
UniProt登记号
运输
dry ice
储存温度
−70°C
基因信息
human ... KDM4B(23030)
一般描述
Human JMJD2B, also known as JHDM3B and KDM4B (GenBank Accession No. NM_015015), amino acids 2-500 with N-terminal GST-tag, MW=83 kDa, expressed in Sf9 cells using a Baculovirus expression system.
特异性
0.32 pmole/min/μg Assay conditions: 10μl reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 50 mM NaCl, 1mM TCEP, 500 μM a-ketoglutarate, 25 μM iron, 2mM ascorbic acid, 0.01% Tween20, 0.5 μM biotinylated peptide substrate, and JMJD2B (2 – 10ng) added to the wells. Add antibody against di-methylated K9 peptide. Incubate for 30 min, then Streptavidin conjugated secondary antibody followed by Alpha Screening detection.
应用
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
外形
Formulated in: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% Glycerol.
储存及稳定性
At least 6 months at –80°C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary, use carrier protein (BSA 0.1 – 0.5%).
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
Sophie Beyer et al.
The Journal of biological chemistry, 283(52), 36542-36552 (2008-11-06)
Posttranslational histone modifications serve to store epigenetic information and control both nucleosome assembly and recruitment of non-histone proteins. Histone methylation occurs on arginine and lysine residues and is involved in the regulation of gene transcription. A dynamic control of these
Barna D Fodor et al.
Genes & development, 20(12), 1557-1562 (2006-06-02)
Histone lysine trimethyl states represent some of the most robust epigenetic modifications in eukaryotic chromatin. Using a candidate approach, we identified the subgroup of murine Jmjd2 proteins to antagonize H3K9me3 at pericentric heterochromatin. H3K27me3 and H4K20me3 marks are not impaired
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