生物来源
human
重组
expressed in baculovirus infected insect cells
方案
≥70% (SDS-PAGE)
表单
aqueous solution
分子量
118 kDa
包装
pkg of 100 μg
储存条件
avoid repeated freeze/thaw cycles
浓度
>0.02 mg/mL
NCBI登记号
UniProt登记号
运输
dry ice
储存温度
−70°C
基因信息
human ... UBA6(55236)
一般描述
Human UBA6 (UBE1L2) (GenBank Accession No. NM_018227), amino acids 2-end, with N-terminal DDDDK tag (FLAG), MW = 118 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
应用
Useful in conjunction with E2 and E3 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
外形
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 100 μg/ml FLAG peptide, 20% glycerol and 3 mM DTT.
制备说明
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
法规信息
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