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安全信息

SRP0276

Sigma-Aldrich

PDE10A Active from mouse

recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

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别名:
Dual-substrate cAMP and cGMP, cyclic nucleotide phosphodiesterase, PDE10A2 brain-specific isoform, Phosphodiesterase 10A
UNSPSC代码:
12352200
NACRES:
NA.32

生物来源

mouse

重组

expressed in baculovirus infected insect cells

检测方案

≥80% (SDS-PAGE)

形式

aqueous solution

分子量

114.5 kDa

包装

pkg of 10 μg

浓度

>0.02 mg/mL

NCBI登记号

UniProt登记号

运输

dry ice

储存温度

−70°C

基因信息

mouse ... PDE10A(23984)

一般描述

Mouse PDE10A GenBank Accession No. NM_011866), full length with N-terminal GST tag, MW = 114.5 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

应用

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

单位定义

One unit is defined as the amount of enzyme that will convert 1 pmole of 3′, 5′-cAMP to 5′ AMP per min at 37°C

外形

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

制备说明

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

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