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生物来源
human
重组
expressed in baculovirus infected insect cells
检测方案
≥70% (SDS-PAGE)
形式
aqueous solution
分子量
88 kDa
包装
pkg of 10 μg
浓度
>0.02 mg/mL
NCBI登记号
UniProt登记号
运输
dry ice
储存温度
−70°C
基因信息
human ... EGFR(1956)
一般描述
Human EGFR L858R (GenBank Accession No. NM_005228), (amino acids 668-1210) with N-terminal GST tag, MW = 88 kDa, expressed in baculovirus expression system.
应用
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
单位定义
One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Tyr substrate per minute at pH 7.4 and 30°C.
外形
Formulated in 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.
制备说明
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
法规信息
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