SRP0229
WWP1 Active human
recombinant, expressed in baculovirus infected insect cells, ≥15% (SDS-PAGE)
别名:
AIP5, Atrophin-1 interacting protein 5, NEDD4-like E3 ubiquitin-protein ligase, TGIF-interacting ubiquitin ligase 1 (Tiul1), WW domain containing E3 ubiquitin protein ligase 1
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About This Item
UNSPSC代码:
12352200
NACRES:
NA.32
推荐产品
生物来源
human
重组
expressed in baculovirus infected insect cells
方案
≥15% (SDS-PAGE)
表单
aqueous solution
分子量
106 kDa
包装
pkg of 20 μg
储存条件
avoid repeated freeze/thaw cycles
浓度
>0.02 mg/mL
NCBI登记号
UniProt登记号
运输
dry ice
储存温度
−70°C
基因信息
human ... WWP1(11059)
一般描述
Human WWP1 (GenBank Accession No. NM_007013), amino acids 2-end, with N-terminal DDDDK tag (FLAG), MW = 106 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
应用
Useful in conjunction with E1 and E2 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
单位定义
One unit is defined as the amount of enzyme required to transfer 1 pmol of ubiquitin to substrate/min at 37°C.
外形
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 100 μg/ml FLAG peptide, 20% glycerol and 3 mM DTT.
制备说明
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
法规信息
常规特殊物品
从最新的版本中选择一种：
Yu-Ru Lee et al.
Science (New York, N.Y.), 364(6441) (2019-05-18)
Activation of tumor suppressors for the treatment of human cancer has been a long sought, yet elusive, strategy. PTEN is a critical tumor suppressive phosphatase that is active in its dimer configuration at the plasma membrane. Polyubiquitination by the ubiquitin
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