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安全信息

SRP0220

Sigma-Aldrich

DEP1 Active human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

别名:

CD148, DEP-1, Density-enhanced phosphatase 1, PTPRJ, Protein tyrosine phosphatase, receptor type, J

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About This Item

UNSPSC代码:
12352200
NACRES:
NA.32

生物来源

human

重组

expressed in E. coli

方案

≥90% (SDS-PAGE)

表单

aqueous solution

效能

≥0.114 units/mg

分子量

58.9 kDa

包装

pkg of 20 μg

浓度

>0.02 mg/mL

NCBI登记号

UniProt登记号

运输

dry ice

储存温度

−70°C

基因信息

human ... PTPRJ(5795)

一般描述

Protein tyrosine phosphatase (density-enhanced phosphatase-1, PTP-eta, CD148), Catalytic domain, (amino acids 1024-1306) (Gene bank accession no. NM_U10886) with N-terminal GST tag, MW=58.9 kDa, expressed in an E. coli expression system.

应用

Useful for the study of enzyme kinetics, regulation, and to dephosphorylate target substrates.

单位定义

One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.

外形

Formulated in 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, and 10 mM glutathione.

制备说明

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

法规信息

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James R Whiteford et al.
Molecular biology of the cell, 22(19), 3609-3624 (2011-08-05)
Syndecan-2 is a heparan sulfate proteoglycan that has a cell adhesion regulatory domain contained within its extracellular core protein. Cell adhesion to the syndecan-2 extracellular domain (S2ED) is β1 integrin dependent; however, syndecan-2 is not an integrin ligand. Here the

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