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生物来源
mouse
重组
expressed in E. coli
方案
≥90% (SDS-PAGE)
表单
aqueous solution
分子量
63.3 kDa
包装
pkg of 20 μg
储存条件
avoid repeated freeze/thaw cycles
浓度
>0.02 mg/mL
NCBI登记号
UniProt登记号
运输
dry ice
储存温度
−70°C
基因信息
human ... PTPN1(5770)
一般描述
Protein tyrosine phosphatase, Catalytic domain, (amino acids 1-321), with N-terminal GST tag, MW = 63.3 kDa, expressed in an E. coli expression system.
应用
Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.
单位定义
One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.
外形
Formulation: recombinant proteins were eluted in 25 mM Tris-HCL, 75 mM NaCl, pH 8.0, 0.05% Tween, 10 mM glutathione, 1 mM DTT and 50% glycerol.
制备说明
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
法规信息
常规特殊物品
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