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安全信息

SRP0213

Sigma-Aldrich

PTP1B (1-321)/PTPN1 Active from mouse

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

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别名:
PTPN1, Protein tyrosine phosphatase, non-receptor type 1
UNSPSC代码:
12352200
NACRES:
NA.32

生物来源

mouse

重组

expressed in E. coli

检测方案

≥90% (SDS-PAGE)

形式

aqueous solution

分子量

63.3 kDa

包装

pkg of 20 μg

储存条件

avoid repeated freeze/thaw cycles

浓度

>0.02 mg/mL

NCBI登记号

UniProt登记号

运输

dry ice

储存温度

−70°C

基因信息

human ... PTPN1(5770)

一般描述

Protein tyrosine phosphatase, Catalytic domain, (amino acids 1-321), with N-terminal GST tag, MW = 63.3 kDa, expressed in an E. coli expression system.

应用

Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.

单位定义

One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.

外形

Formulation: recombinant proteins were eluted in 25 mM Tris-HCL, 75 mM NaCl, pH 8.0, 0.05% Tween, 10 mM glutathione, 1 mM DTT and 50% glycerol.

制备说明

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

法规信息

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