生物来源
human
重组
expressed in baculovirus infected insect cells
检测方案
≥80% (SDS-PAGE)
形式
aqueous solution
分子量
65.6 kDa
包装
pkg of 10 μg
浓度
>0.02 mg/mL
NCBI登记号
UniProt登记号
运输
dry ice
储存温度
−70°C
基因信息
human ... PARP11(57097)
一般描述
Human PARP11 (GenBank Accession No. NM_020367), full length with N-terminal GST tag, MW = 65.6 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
应用
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
单位定义
One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute from NAD into acid-insoluble form.
外形
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.
制备说明
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
WGK
WGK 1
法规信息
常规特殊物品
Mirella L Meyer-Ficca et al.
Biology of reproduction, 92(3), 80-80 (2015-02-13)
Sperm are highly differentiated cells characterized by their species-specific nuclear shapes and extremely condensed chromatin. Abnormal head shapes represent a form of teratozoospermia that can impair fertilization capacity. This study shows that poly(ADP-ribose) polymerase-11 (ARTD11/PARP11), a member of the ADP-ribosyltransferase
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