SRP0151
SMYD2 Active human
recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)
别名:
KMT3C, Lysine N-methyltransferase 3C, SET and MYND domain containing 2, ZMYND14, Zinc finger, MYND domain containing 14
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关于此项目
NACRES:
NA.32
UNSPSC Code:
12352200
Form:
aqueous solution
Assay:
≥80% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in baculovirus infected insect cells
Mol wt:
49.7 kDa
产品名称
SMYD2 Active human, recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)
biological source
human
recombinant
expressed in baculovirus infected insect cells
assay
≥80% (SDS-PAGE)
form
aqueous solution
mol wt
49.7 kDa
packaging
pkg of 20 μg
storage condition
avoid repeated freeze/thaw cycles
concentration
>0.02 mg/mL
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... SMYD2(56950)
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Biochem/physiol Actions
SET and MYND domain containing 2 (SMYD2) might act as a putative oncogene by methylating p53 and reducing its tumor suppressive role. Overexpression of SMYD2 gene is associated with pathogenesis and metastasis of oesophageal cancer, squamous cell carcinoma (ESCC). Therefore, it is considered as a potent therapeutic target for ESCC. SMYD2 has capacity to catalyze the methylation of histone and non-histone substrates. AZ505 acts as a potent and selective inhibitor of SMYD2 and aids in the improvement of novel cancer treatment.
General description
SET and MYND domain containing 2 (SMYD2) is encoded by the gene mapped to human chromosome 1q32.3-q41. The encoded protein belongs to SMYD family of protein lysine methyltransferases.
Human SMYD2 (GenBank Accession No. NM_020197), full length with N-terminal DDDDK tag (FLAG), MW = 49.7kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Human SMYD2 (GenBank Accession No. NM_020197), full length with N-terminal DDDDK tag (FLAG), MW = 49.7kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 100 μg/ml FLAG peptide, 20% glycerol and 3 mM DTT.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
存储类别
10 - Combustible liquids
wgk
WGK 2
法规信息
新产品
此项目有
Repression of p53 activity by Smyd2-mediated methylation.
Huang J
Nature, 444(7119), 629-632 (2006)
Makoto Nakakido et al.
Neoplasia (New York, N.Y.), 17(4), 367-373 (2015-05-01)
Phosphatase and tensin homologue (PTEN), one of the well-characterized tumor suppressor proteins, counteracts the phosphatidylinositol 3-kinase-AKT pathway through its unique lipid phosphatase activity. The functions of PTEN are regulated by a variety of posttranslational modifications such as acetylation, oxidation, ubiquitylation
Overexpression of SMYD2 relates to tumor cell proliferation and malignant outcome of esophageal squamous cell carcinoma.
Komatsu S
Carcinogenesis, 30(7), 1139-1146 (2009)
Prediction of CCND1 amplification using plasma DNA as a prognostic marker in oesophageal squamous cell carcinoma.
Takeshita H
British Journal of Cancer, 102(9), 1378-1383 (2010)
Structural basis of substrate methylation and inhibition of SMYD2.
Ferguson AD
Structure, 19(9), 1262-1273 (2011)
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