生物来源
human
重组
expressed in baculovirus infected insect cells
方案
≥80% (SDS-PAGE)
表单
aqueous solution
分子量
71 kDa
包装
pkg of 10 μg
浓度
>0.02 mg/mL
NCBI登记号
UniProt登记号
运输
dry ice
储存温度
−70°C
基因信息
human ... TRDMT1(1787)
一般描述
DNA (cytosine-5-)-methyltransferase 2 (DNMT2) is a highly conserved protein which is part of the DNA methyltransferase family. It possesses a conserved cysteine residue in its catalytic pocket. The gene encoding it is localized on human chromosome 10p14->p12.
应用
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
生化/生理作用
DNA (cytosine-5-)-methyltransferase 2 (DNMT2) methylates the cytosine-38 residue of the aspartic acid transfer RNA (tRNA-Asp). It has been shown to interact with the anticodon stem and loop. The protein also functions in cellular physiology and stress response. It is significantly expressed in cancers.
外形
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 10% glycerol.
制备说明
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
法规信息
新产品
从最新的版本中选择一种：
Azacytidine Inhibits RNA Methylation at DNMT2 Target Sites in Human Cancer Cell Lines
Matthias Schaefer
Cancer Research, 69(20) (2009)
Mapping the tRNA binding site on the surface of human DNMT2 methyltransferase.
Jurkowski TP
Biochemistry, 51(22), 4438-4444 (2012)
Assignment1 of candidate DNA methyltransferase gene (DNMT2) to human chromosome band 10p15.1 by in situ hybridization
Vilain A
Cytogenetic and genome research, 82 (1998)
Human DNMT2 methylates tRNAAsp molecules using a DNA methyltransferase-like catalytic mechanism
Tomasz P. Jurkowski
RNA, 14(8), 1663-1670 (2008)
Winfried Elhardt et al.
Biochimie, 112, 66-72 (2015-03-10)
Methylation of tRNA is an important post-transcriptional modification and aberrations in tRNA modification has been implicated in cancer. The DNMT2 protein methylates C38 of tRNA-Asp and it has a role in cellular physiology and stress response and its expression levels
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门