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安全信息

SRP0125

Sigma-Aldrich

LSD1 substrate (Di-methylated K4_H3)

≥90% (HPLC), aqueous solution

别名:

HIST1H3E, Histone H3.1

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About This Item

UNSPSC代码:
12352204
NACRES:
NA.32

product name

LSD1 substrate (Di-methylated K4_H3), ≥90% (HPLC)

生物来源

human

检测方案

≥90% (HPLC)

形式

aqueous solution

分子量

2283 Da

包装

pkg of 500UL

浓度

0.55 mg/mL

UniProt登记号

运输

dry ice

储存温度

−70°C

基因信息

human ... HIST1H3E(8353)

一般描述

Lysine-specific demethylase 1 (LSD1) acts on mono- and dimethylated histones and demethylates them. It has a role in embryogenesis, cell proliferation, spermatogenesis, adipogenesis and chromosomal segregation. Histone H3 peptide methylated at Lysine 4 is a substrate for LSD1. Histone cluster 1 H3 family member E (HIST1H3E) is a variant of histone 3 and is also called as the replicative histone. It is mainly expressed in the S-phase of the cell cycle. The gene encoding this protein is localized on human chromosome 6p22.

应用

Study enzyme kinetics and screen small molecular inhibitors of LSD1 for drug discovery and HTS applications

生化/生理作用

Histones have an important role in the organization and modification of chromatin. Histone cluster 1 H3 family member E (HIST1H3E) has a role in DNA synthesis during DNA replication and might also be involved in DNA repair. It is deposited at the chromatin at the time of DNA replication-associated chromatin assembly. During herpes simplex virus 1 (HSV-1) infection, differential movement of HIST1H3E is associated with the assembly of viral chromatin.

外形

Supplied as 500 uL of water solution at 200 uM concetration.

制备说明

Thaw on ice. Upon first thaw, briefly spin tube to recover full content of the tube. Aliquot into single use aliquots. Store remaining product in aliquots at -70°C. Note: Avoid freeze/thaw cycles.

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

分析证书(COA)

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the sequence of the peptide for Product SRP0125, LSD1 substrate (Di-methylated K4_H3)?

    Product SRP0125 is a 21 amino acid peptide. The sequence is:ARTK*QTARKSTGGKAPRKQLA, where K* is dimethylated lysine.The sequence of the peptide corresponds to the N-terminal (minus the methionine) portion of human Histone H3, with the K4 (lysine at position 4) dimethylated.

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Yingwei Chen et al.
Critical reviews in eukaryotic gene expression, 22(1), 53-59 (2012-02-22)
Lysine-specific demethylase 1 (LSD1), the first identified histone demethylase, was belonged to the superfamily of the flavin adenine dinucleotide (FAD)-dependent amine oxidases. LSD1 specifically demethylates mono- or dimethylated dimethylated histone H3 lysine4 (H3K4) and H3 lysine 9 (H3K9) via a
Kami Ahmad et al.
Molecular cell, 9(6), 1191-1200 (2002-06-28)
Two very similar H3 histones-differing at only four amino acid positions-are produced in Drosophila cells. Here we describe a mechanism of chromatin regulation whereby the variant H3.3 is deposited at particular loci, including active rDNA arrays. While the major H3
Kristen L Conn et al.
PLoS pathogens, 9(10), e1003695-e1003695 (2013-10-17)
During lytic infections, HSV-1 genomes are assembled into unstable nucleosomes. The histones required for HSV-1 chromatin assembly, however, are in the cellular chromatin. We have shown that linker (H1) and core (H2B and H4) histones are mobilized during HSV-1 infection
Eric I Campos et al.
Nature structural & molecular biology, 17(11), 1343-1351 (2010-10-19)
The mechanism by which newly synthesized histones are imported into the nucleus and deposited onto replicating chromatin alongside segregating nucleosomal counterparts is poorly understood, yet this program is expected to bear on the putative epigenetic nature of histone post-translational modifications.
Hideaki Tagami et al.
Cell, 116(1), 51-61 (2004-01-14)
Deposition of the major histone H3 (H3.1) is coupled to DNA synthesis during DNA replication and possibly DNA repair, whereas histone variant H3.3 serves as the replacement variant for the DNA-synthesis-independent deposition pathway. To address how histones H3.1 and H3.3

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