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安全信息

SRE0119

Sigma-Aldrich

dsDNase

For gDNA removal, recombinant, expressed in Pichia pastoris, Heat labile, Triton-free

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别名:
HL-dsDNase, heat labile double strand specific deoxyribonuclease
UNSPSC代码:
12352204
NACRES:
NA.77

重组

expressed in Pichia pastoris

质量水平

组成

Triton-free

包装

pkg of 250 U

运输

dry ice

储存温度

−20°C

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一般描述

Double-strand specific DNases (dsDNases) are unique double-strand specific endonucleases. As they do not digest ssDNA or RNA, they can be used to specifically remove dsDNA in the presence of other nucleic acids.

应用

This heat labile dsDNase removes genomic DNA from RNA preparations with minimal impact on RNA integrity.with minimal impact on RNA integrity

特点和优势

Due to the mild inactivation temperature required, this dsDNase can remove genomic DNA from RNA perparations and have minimal impact onto RNA quality and quantity.

单位定义

One unit is defined as an increase in absorbance at 260 nm of 0.001 per minute, using 50 µg/ml of high MW DNA in 100 mM Na-acetate pH 5.0 and 5 mM MgCl2.

重悬

In solution

其他说明

The heat labile dsDNase is highly active in a temperature range of 20-40°C. It needs at least 2.5 mM Mg for activity and has an optimal pH at 7.5.

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

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