应用
Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.1. Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation.
不同种类的马铃薯(S. tuberosum)中存在至少2种同工酶：一种的ATP酶/ADP酶比例较高(∼10)和另一种较低(∼1)。
反应：ATP → ADP+Pi → AMP+2Pi。
反应：ATP → ADP+Pi → AMP+2Pi。
生化/生理作用
Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis. Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM.
单位定义
One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.
外形
Partially purified, lyophilized powder containing potassium succinate buffer salts.
制备说明
按 ATP 酶单位销售。
其他说明
该等级具有低 ATP 酶/ADP 酶比率。
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物源性产品
Kettlun, A., et al.
Phytochemistry, 21, 551-551 (1982)
David Riewe et al.
Plant physiology, 147(3), 1092-1109 (2008-05-16)
Apyrases hydrolyze nucleoside triphosphates and diphosphates and are found in all eukaryotes and a few prokaryotes. Although their enzymatic properties have been well characterized, relatively little is known regarding their subcellular localization and physiological function in plants. In this study
Differentiation of platelet-aggregating effects of human tumor cell lines based on inhibition studies with apyrase, hirudin, and phospholipase.
E Bastida et al.
Cancer research, 42(11), 4348-4352 (1982-11-01)
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