SML2135
生物素基酪酰胺
≥97% (HPLC), powder, tyramide signal amplifier
别名:
生物素酚, （3AS，4S，6AR）-六氢-N- [2-（4-羟基苯基）乙基] -2-氧代-1H-噻吩并[3，4-d]咪唑-4-戊
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关于此项目
经验公式（希尔记法）:
C18H25N3O3S
化学文摘社编号:
分子量:
363.47
UNSPSC Code:
12352200
NACRES:
NA.77
MDL number:
产品名称
生物素基酪酰胺, ≥97% (HPLC)
InChI
1S/C18H25N3O3S/c22-13-7-5-12(6-8-13)9-10-19-16(23)4-2-1-3-15-17-14(11-25-15)20-18(24)21-17/h5-8,14-15,17,22H,1-4,9-11H2,(H,19,23)(H2,20,21,24)/t14-,15-,17-/m0/s1
InChI key
VZWXNOBHWODXCW-ZOBUZTSGSA-N
SMILES string
O=C(NCCC1=CC=C(O)C=C1)CCCC[C@@H]2SC[C@@]3([H])[C@]2([H])NC(N3)=O
assay
≥97% (HPLC)
form
powder
color
white to beige
solubility
DMSO: 2 mg/mL, clear
storage temp.
−20°C
相关类别
Application
生物素酪胺或生物素-苯酚已用于邻近标记法蛋白质鉴定。
Biochem/physiol Actions
生物素酪胺是一种酪胺信号放大试剂，适于免疫组织化学(IHC)和原位杂交实验，以及显色和荧光检测。 探针初步结合之后，接着用 HRP 标记的抗体或链霉抗生物素蛋白缀合物进行二次探针检测。 HRP 的催化作用导致在探针附近沉积多个生物素基酪酰胺分子，然后可以用标记的链霉抗生物素蛋白缀合物进行检测。 检测灵敏度可以是传统检测方法的100倍或更高。
用于酪胺信号放大的试剂
存储类别
11 - Combustible Solids
wgk
WGK 3
J F Breininger et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 48(12), 1593-1599 (2000-12-02)
To increase the sensitivity of fluorescence in situ hybridization (FISH) for detection of low-abundance mRNAs, we performed FISH on cryostat sections of rat hypothalamus with biotin-labeled riboprobes to leptin receptor (ObRb) and amplified the signal by combining tyramide signal amplification
Nguyen Thi Trang et al.
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, 27(3), 326-331 (2015-04-10)
This study evaluated the sensitivity of biotinyl-tyramide-based in situ hybridization (TISH) method by comparison with chromogenic in situ hybridization (CISH) and immunohistochemical staining (IHC) methods. This study also determined the effect of fixative and fixation time on the detection of
Xiangru Huo et al.
Molecular cell, 77(2), 368-383 (2019-11-05)
Interphase chromatin is hierarchically organized into higher-order architectures that are essential for gene functions, yet the biomolecules that regulate these 3D architectures remain poorly understood. Here, we show that scaffold attachment factor B (SAFB), a nuclear matrix (NM)-associated protein with
Marina Yu Mazina et al.
Scientific reports, 10(1), 4793-4793 (2020-03-18)
Proximity-dependent biotin labelling revealed undescribed participants of the ecdysone response in Drosophila. Two labelling enzymes (BioID2 and APEX2) were fused to EcR or Usp to biotin label the surrounding proteins. The EcR/Usp heterodimer was found to collaborate with nuclear pore
Misuzu Kurihara et al.
Molecular cell, 78(3), 493-505 (2020-05-01)
The promyelocytic leukemia (PML) body is a phase-separated nuclear structure physically associated with chromatin, implying its crucial roles in genome functions. However, its role in transcriptional regulation is largely unknown. We developed APEX-mediated chromatin labeling and purification (ALaP) to identify
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