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检测方案
≥97% (HPLC)
形式
powder
颜色
white to beige
溶解性
DMSO: 2 mg/mL, clear
储存温度
−20°C
SMILES字符串
O=C(NCCC1=CC=C(O)C=C1)CCCC[C@@H]2SC[C@@]3([H])[C@]2([H])NC(N3)=O
应用
Biotinyl tyramide or Biotin-Phenol has been used in proximity-dependent labeling for protein identification.
生化/生理作用
生物素基酪酰胺一种用于酪胺信号放大的试剂，用于免疫组织化学（IHC）和原位杂交方案，以及通过发色或荧光检测。 探针初步结合之后，接着用 HRP 标记的抗体或链霉抗生物素蛋白缀合物进行二次探针检测。 HRP 的催化作用导致在探针附近沉积多个生物素基酪酰胺分子，然后可以用标记的链霉抗生物素蛋白缀合物进行检测。 检测灵敏度可以是传统检测方法的 100 倍或更高。
WGK
WGK 3
J F Breininger et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 48(12), 1593-1599 (2000-12-02)
To increase the sensitivity of fluorescence in situ hybridization (FISH) for detection of low-abundance mRNAs, we performed FISH on cryostat sections of rat hypothalamus with biotin-labeled riboprobes to leptin receptor (ObRb) and amplified the signal by combining tyramide signal amplification
Nguyen Thi Trang et al.
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, 27(3), 326-331 (2015-04-10)
This study evaluated the sensitivity of biotinyl-tyramide-based in situ hybridization (TISH) method by comparison with chromogenic in situ hybridization (CISH) and immunohistochemical staining (IHC) methods. This study also determined the effect of fixative and fixation time on the detection of
Xiangru Huo et al.
Molecular cell, 77(2), 368-383 (2019-11-05)
Interphase chromatin is hierarchically organized into higher-order architectures that are essential for gene functions, yet the biomolecules that regulate these 3D architectures remain poorly understood. Here, we show that scaffold attachment factor B (SAFB), a nuclear matrix (NM)-associated protein with
Hagai Marmor-Kollet et al.
Molecular cell, 80(5), 876-891 (2020-11-21)
Stress granules (SGs) are cytoplasmic assemblies of proteins and non-translating mRNAs. Whereas much has been learned about SG formation, a major gap remains in understanding the compositional changes SGs undergo during normal disassembly and under disease conditions. Here, we address
Peiguo Yang et al.
Cell, 181(2), 325-345 (2020-04-18)
The mechanisms underlying ribonucleoprotein (RNP) granule assembly, including the basis for establishing and maintaining RNP granules with distinct composition, are unknown. One prominent type of RNP granule is the stress granule (SG), a dynamic and reversible cytoplasmic assembly formed in
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