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产品名称
诺考达唑制备液, 5 mg/mL, DMSO solution
生物来源
synthetic
质量水平
表单
DMSO solution
浓度
5 mg/mL
运输
dry ice
储存温度
−20°C
SMILES字符串
[s]1c(ccc1)C(=O)c2cc3nc([nH]c3cc2)NC(=O)OC
InChI
1S/C14H11N3O3S/c1-20-14(19)17-13-15-9-5-4-8(7-10(9)16-13)12(18)11-3-2-6-21-11/h2-7H,1H3,(H2,15,16,17,19)
InChI key
KYRVNWMVYQXFEU-UHFFFAOYSA-N
生化/生理作用
诺考达唑是一种抗癌药物，已被证明会干扰间期和有丝分裂细胞中微管的结构和功能。
诺考达唑是一种抗癌药物，已被证明会干扰间期和有丝分裂细胞中微管的结构和功能。恶性细胞可能比非恶性细胞对诺考达唑的抗微管效应更敏感。体外培养的哺乳动物细胞 可用 0.04-10μg/mL 剂量进行细胞同步化实验。诺考达唑处理导致的细胞在有丝分裂中长时间停滞通常导致细胞凋亡死亡。由于不溶性，无法使用较高浓度。操作的高特异性可解释为什么对骨髓细胞毒性低和无神经毒性。诺考达唑被认为可直接与微管蛋白结合，引起构象变化，导致一些巯基和可能的酪氨酸残基暴露增加。诺考达唑与阿糖胞苷在 L1210 白血病细胞上表现出明显的协同作用。
制备说明
诺考达唑在冷冻溶液中形成沉淀，因此解冻后应进行超声处理，使溶液均匀。建议解冻后超声 1-3 分钟，以除去可能因冷冻而出现的沉淀。
对于细胞培养工作，用培养基稀释诺考达唑现成溶液 (5 mg/mL DMSO)，稀释溶液在生物学中至少可稳定 7 天。体外培养的哺乳动物细胞 可用 0.04-10μg/mL 剂量。
对于细胞培养工作，用培养基稀释诺考达唑现成溶液 (5 mg/mL DMSO)，稀释溶液在生物学中至少可稳定 7 天。体外培养的哺乳动物细胞 可用 0.04-10μg/mL 剂量。
储存及稳定性
本品在-20C 下可稳定保存 2 年。
储存分类代码
10 - Combustible liquids
WGK
WGK 2
闪点(°F)
188.6 °F - closed cup
闪点(°C)
87 °C - closed cup
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Francesco Cambuli et al.
EMBO reports, 23(5), e54049-e54049 (2022-03-08)
The healthy prostate is a relatively quiescent tissue. Yet, prostate epithelium overgrowth is a common condition during aging, associated with urinary dysfunction and tumorigenesis. For over thirty years, TGF-β ligands have been known to induce cytostasis in a variety of
Anne Meldgaard Hansen et al.
Science advances, 8(11), eabf8627-eabf8627 (2022-03-19)
Activation of interferon genes constitutes an important anticancer pathway able to restrict proliferation of cancer cells. Here, we demonstrate that the H3K9me3 histone methyltransferase (HMT) suppressor of variegation 3-9 homolog 1 (SUV39H1) is required for the proliferation of acute myeloid
Juan A Cantres-Velez et al.
Molecular and cellular biology, 41(8), e0023421-e0023421 (2021-06-08)
Fanconi anemia (FA) is a rare genetic disease characterized by increased risk for bone marrow failure and cancer. The FA proteins function together to repair damaged DNA. A central step in the activation of the FA pathway is the monoubiquitination
Dhivya Kumar et al.
The Journal of cell biology, 220(9) (2021-07-10)
Cells inherit two centrioles, the older of which is uniquely capable of generating a cilium. Using proteomics and superresolved imaging, we identify a module that we term DISCO (distal centriole complex). The DISCO components CEP90, MNR, and OFD1 underlie human
Jean Defourny et al.
Hearing research, 400, 108137-108137 (2020-12-09)
In the cochlea, connexins 26 (Cx26) and 30 (Cx30) largely co-assemble into heteromeric gap junctions, which connect adjacent non-sensory epithelial cells. These channels are believed to ensure the rapid removal of K+ away from the base of sensory hair cells
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