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生物来源
synthetic
质量水平
形式
DMSO solution
浓度
5 mg/mL
运输
dry ice
储存温度
−20°C
InChI
1S/C14H11N3O3S/c1-20-14(19)17-13-15-9-5-4-8(7-10(9)16-13)12(18)11-3-2-6-21-11/h2-7H,1H3,(H2,15,16,17,19)
InChI key
KYRVNWMVYQXFEU-UHFFFAOYSA-N
生化/生理作用
诺考达唑是一种抗癌药物，已被证明会干扰间期和有丝分裂细胞中微管的结构和功能。恶性细胞可能比非恶性细胞对诺考达唑的抗微管效应更敏感。体外培养的哺乳动物细胞 可用 0.04-10μg/mL 剂量进行细胞同步化实验。诺考达唑处理导致的细胞在有丝分裂中长时间停滞通常导致细胞凋亡死亡。由于不溶性，无法使用较高浓度。操作的高特异性可解释为什么对骨髓细胞毒性低和无神经毒性。诺考达唑被认为可直接与微管蛋白结合，引起构象变化，导致一些巯基和可能的酪氨酸残基暴露增加。诺考达唑与阿糖胞苷在 L1210 白血病细胞上表现出明显的协同作用。
制备说明
诺考达唑在冷冻溶液中形成沉淀，因此解冻后应进行超声处理，使溶液均匀。建议解冻后超声 1-3 分钟，以除去可能因冷冻而出现的沉淀。
对于细胞培养工作，用培养基稀释诺考达唑现成溶液 (5 mg/mL DMSO)，稀释溶液在生物学中至少可稳定 7 天。体外培养的哺乳动物细胞 可用 0.04-10μg/mL 剂量。
对于细胞培养工作，用培养基稀释诺考达唑现成溶液 (5 mg/mL DMSO)，稀释溶液在生物学中至少可稳定 7 天。体外培养的哺乳动物细胞 可用 0.04-10μg/mL 剂量。
储存及稳定性
本品在-20C 下可稳定保存 2 年。
WGK
WGK 2
闪点(°F)
188.6 °F
闪点(°C)
87 °C
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