SML1661
Bafilomycin A1
from Streptomyces griseus, ≥90% (HPLC), DMSO solution, V-ATPase inhibitor
别名:
BafA1
产品名称
Bafilomycin A1 Ready Made Solution, 0.16 mM in DMSO, from Streptomyces griseus
SMILES string
O1[C@@H]([C@H]([C@@H](C[C@]1(O)[C@H]([C@H](O)[C@@H]([C@H]2OC(=O)\C(=C\C(=C\[C@H]([C@H]([C@H](C\C(=C\C=C\[C@@H]2OC)\C)C)O)C)\C)\OC)C)C)O)C)C(C)C
InChI
1S/C35H58O9/c1-19(2)32-24(7)27(36)18-35(40,44-32)26(9)31(38)25(8)33-28(41-10)14-12-13-20(3)15-22(5)30(37)23(6)16-21(4)17-29(42-11)34(39)43-33/h12-14,16-17,19,22-28,30-33,36-38,40H,15,18H2,1-11H3/b14-12+,20-13+,21-16+,29-17-/t22-,23+,24-,25-,26-,27+,28-,30-,31+,32+,33+,35+/m0/s1
InChI key
XDHNQDDQEHDUTM-JQWOJBOSSA-N
biological source
Streptomyces griseus
form
DMSO solution
concentration
0.16 mM in DMSO
shipped in
dry ice
storage temp.
−20°C
Quality Level
Other Notes
Bafilomycin A1 solution is provided in concentration of 0.16 mM. Typical concentrations for use in cell culture are 50-100 nM.
Application
Bafilomycin A1 has been used:
- as an endosome acidification inhibitor to study the importance of endosome acidification in the extracellular vesicle uptake and cytosolic release of stably expressing NanoLuc luciferase-tagged Hsp70 (NLuc-Hsp70) in HeLa cells
- as a vacuolar-type H+-ATPase (V-ATPase) inhibitor to study its effects on autophagic turnover of light chain 3 β (LC3-II) in mice
- as an autophagy inhibitor to study its effects on primary rat liver sinusoidal endothelial cells (LSECs) defenestration
Biochem/physiol Actions
Bafilomycin A1 inhibits autophagy. It may exhibit anti-tumorigenic, anti-parasitic, and anti-neurodegenerative effects. Bafilomycin A1, found in lysosomes and endosomes prevents the acidification of these cell organelles. It also participates in blocking autophagosome-lysosome fusion and autolysosome acidification, steps necessary for maintaining the autophagic flux and cellular homeostasis.
Bafilomycin A1 is a macrolide antibiotic.
Bafilomycin A1 is a macrolide antibiotic. Bafilomycin A1 acts as a potent and selective inhibitor of vacuolar-type H+-ATPase.
General description
Bafilomycin A1, a macrolide antibiotic, belongs to the pleomacrolides family. It acts as a potent and selective inhibitor of vacuolar-type H+-ATPase. BafA1 can inhibit the viability of MG63 osteosarcoma cells. It can also stimulate mitochondrial dysfunction. BafA1 inhibits the proliferation of different types of cancer cells.
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
188.6 °F - closed cup
flash_point_c
87 °C - closed cup
法规信息
新产品
此项目有
Molecular basis of V-ATPase inhibition by bafilomycin A1
Wang R, et al.
Nature Communications (2021)
Bafilomycin A1 inhibits autophagy and induces apoptosis in MG63 osteosarcoma cells
Xie Z, et al.
Molecular medicine reports (2014)
Bafilomycin A1 induces caspase-independent cell death in hepatocellular carcinoma cells via targeting of autophagy and MAPK pathways
Yan Y, et al.
Scientific Reports (2016)
Xiaoying Luo et al.
Cell death & disease, 9(5), 576-576 (2018-05-16)
Autophagy, interacting with actin cytoskeleton and the NO-dependent pathway, may affect the phenotype and function of endothelial cells. Moreover, caveolin-1 (Cav-1), as a structure protein in liver sinusoidal endothelial cells (LSECs), is closely related to autophagy. Hence, we aim to
Caroline Mauvezin et al.
Autophagy, 11(8), 1437-1438 (2015-07-15)
Autophagosome-lysosome fusion and autolysosome acidification constitute late steps in the autophagic process necessary to maintain functional autophagic flux and cellular homeostasis. Both of these steps are disrupted by the V-ATPase inhibitor bafilomycin A1, but the mechanisms potentially linking them are
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