SHC004H
MISSION®
High Titer, shRNA sequence targeting tGFP
别名:
MISSION®, MISSION® Control Transduction Particles
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选择尺寸
500 G
CN¥922.59
1 KG
CN¥1,016.24
2.5 KG
CN¥1,757.85
6 X 500 G
CN¥2,787.10
5 KG
CN¥3,039.60
12 KG
CN¥4,266.45
6 X 1 KG
CN¥4,733.38
4 X 2.5 KG
CN¥4,823.57
25 KG
CN¥8,527.66
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选择尺寸
变更视图
500 G
CN¥922.59
1 KG
CN¥1,016.24
2.5 KG
CN¥1,757.85
6 X 500 G
CN¥2,787.10
5 KG
CN¥3,039.60
12 KG
CN¥4,266.45
6 X 1 KG
CN¥4,733.38
4 X 2.5 KG
CN¥4,823.57
25 KG
CN¥8,527.66
About This Item
UNSPSC代码:
41106609
NACRES:
NA.51
推荐产品
产品线
MISSION®
浓度
≥1x109 VP/ml (via p24 assay)
技术
capture ELISA: 109 TU/mL using p24
运输
dry ice
储存温度
−70°C
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此商品 | 798592 | 238597 | SX0760E |
|---|---|---|---|
| assay ≥99% | assay ≥99% | assay ≥99.0% | assay ≥99.0% |
| Quality Level 100 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| grade ACS reagent, anhydrous | grade ACS reagent, anhydrous | grade ACS reagent, anhydrous | grade - |
| mp 884 °C (lit.) | mp 884 °C (lit.) | mp 884 °C (lit.) | mp 884 °C (lit.) |
| pH 5.2-9.2 (25 °C, 5%) | pH 5.2-9.2 (25 °C, 5%) | pH 5.2-9.2 (25 °C, 5% in water) | pH 5.2-8.0 (20 °C, 50 g/L in H2O) |
一般描述
当使用MISSION® TRC shRNA克隆进行实验时，选择适当对照品是您的实验设计的关键要素，以便准确解释敲低结果。 MISSION对照转导颗粒是监测转导效率的关键阳性对照。
想要查看更多应用数据、实验方案和载体图谱，请访问 sigma.com/shrna。
想要查看更多应用数据、实验方案和载体图谱，请访问 sigma.com/shrna。
The MISSION® TurboGFP™ shRNA Control Transduction Particles contain an shRNA sequence targeting TurboGFP. The TurboGFP shRNA Control Particles are useful as a positive knockdown control in experiments using the MISSION TurboGFP positive control vector or in cell lines expressing TurboGFP. TurboGFP is an improved variant of the green fluorescent protein copGFP cloned from copepoda Pontellina plumata.
The TurboGFP shRNA control transduction particles are produced from the sequence-verified lentiviral plasmid, pLKO.1-puro-TurboGFP shRNA (SHC004). Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. In addition, the Control Transduction Particles are pseudotyped with an envelope G glycoprotein from Vesicular Stomatitis Virus (VSV-G), allowing transduction of a wide variety of mammalian cells. 200 μl of 109 TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.
This high titer offering is recommended for customers: who need to utilize smaller volumes of virus, who have very difficult to transduce cell lines, or who need to transduce large numbers of cells.
The TurboGFP shRNA control transduction particles are produced from the sequence-verified lentiviral plasmid, pLKO.1-puro-TurboGFP shRNA (SHC004). Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. In addition, the Control Transduction Particles are pseudotyped with an envelope G glycoprotein from Vesicular Stomatitis Virus (VSV-G), allowing transduction of a wide variety of mammalian cells. 200 μl of 109 TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.
This high titer offering is recommended for customers: who need to utilize smaller volumes of virus, who have very difficult to transduce cell lines, or who need to transduce large numbers of cells.
应用
To see more application data, protocols, vector maps visit sigma.com/shrna.
组分
High titer controls are provided as 10 x 20 μl aliquots.
法律信息
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
TurboGFP is a trademark of Evrogen Co.
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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