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Merck
CN
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文件

安全信息

SEP060

Millipore

Seppro® Supermix

LC5

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UNSPSC代码:
12352200

柱床体积

5 mL

色谱柱直径 × 长度

12.7 mm × 39.5 mm

容量

loading(Flow Through volume from IgY 14 LC10 (SEP040))

储存温度

2-8°C

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一般描述

免疫亲和性分离高丰度蛋白(HAP)已被证明是克服蛋白质浓度宽动态范围和实现低丰度蛋白(LAP)检测最有效的方法之一。鸡的多克隆IgY（蛋黄免疫球蛋白）抗体具有独特的优越特性，可对复杂的蛋白质混合物进行高特异性分离。IgY12柱可有效去除血浆中的12种HAP。去除HAP后，中等丰度蛋白(MAP)是获得LAP的另一个障碍，而LAP是临床重要标志物，也是诸多生物学研究领域的热点问题。因此，若想进行有效和精确的LAP检测分析，MAP分离将是其面临的一个全新挑战。

为应对这一挑战，我们进一步开发了SuperMix IgY系统进一步将样品中的MAP去除。SuperMix系统中含有特异结合中等丰度蛋白的IgY抗体，总蛋白样品通过IgY12或IgY14柱后的流出组分再次通过SuperMix柱子时，MAP被结合到柱子上，流出部分中主要是低丰度蛋白，从而实现对LAP的高度富集。

应用

Seppro® Supermix, LC5（5mL 柱）适用于将人血清中等丰度蛋白(MAP)从人血清低丰度蛋白(LAP)中分离。Seppro® IgY12 或 IgY14亲和性分离去除特异性人血清高丰度蛋白(HAP)。

其他说明

用于去除人血清或血浆中的中等丰度蛋白

法律信息

Seppro is a registered trademark of Merck KGaA, Darmstadt, Germany

试剂盒组分也可单独购买

产品编号
说明
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WGK

WGK 2

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  1. How does the storage temperature relate to shipping conditions?

    The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. See Shipping and Storage for more information.

  2. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  3. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  4. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  5. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  6. My protein sample contains SDS.  Is this compatible with Product SEP060, Seppro® Supermix LC5?  

    No. We do not recommend using protein samples containing SDS with Product No. SEP060. SDS is a detergent and would be expected to affect adversely the activity of the conjugated antibody in terms of depletion.

  7. I originally purchased my Seppro SuperMix LC5 column from Genway.  Can I still contact Genway for technical support?

    All inquiries on the Seppro technology are now to be referred to Sigma-Aldrich. Customers should not contact Genway for technical assistance.

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Chien-Lun Chen et al.
Journal of proteomics, 85, 28-43 (2013-05-02)
In this study, we evaluated the reproducibility of abundant urine protein depletion by hexapeptide-based library beads and an antibody-based affinity column using the iTRAQ technique. The antibody-based affinity-depletion approach, which proved superior, was then applied in conjunction with iTRAQ to
Majlinda Kullolli et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 939, 10-16 (2013-10-05)
Human plasma is a commonly used diagnostic fluid in clinical chemistry. In-depth plasma proteomic analysis is performed to search for disease biomarkers, however the large dynamic range of protein abundance in plasma presents a substantial analytical challenge. Removal of abundant

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