产品名称
塞普罗 ™ IgY14, Spin Columns
capacity
1 mg total protein loading(14 μL of human plasma, average human protein concentration 70 mg/mL)
storage temp.
2-8°C
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Application
Seppro™ IgY14，离心柱（吸附能力：15-20μl）基于免疫亲和作用，是专门的血浆/血清蛋白去除工具，可通过液相色谱法去除人血浆蛋白混合物中的14种高丰度蛋白(HAP)。Seppro™ IgY14完美支持对人血清/血浆蛋白中低丰度蛋白的分析。
General description
Seppro™ IgY14 柱可从血浆蛋白混合物中去除14种高丰度蛋白。与同类产品相比，鸡的多克隆IgY（蛋黄免疫球蛋白）抗体具有显著的高特异性分离优势，可为研究者提供更干净的人蛋白质样品。Seppro™ 柱帮助研究人员更加简单的完成对血浆蛋白质组的进一步蛋白质组学分析，并且获得更为清晰的结果。
可去除的蛋白质：
白蛋白
IgG
α1-抗胰酶蛋白
IgA
IgM
转铁蛋白
触珠蛋白
α2-巨球蛋白
纤维蛋白原
补体蛋白C3
α1-酸性糖蛋白 (血清类粘蛋白)
HDL (载脂蛋白A-I 和A-II)
LDL (主要是载脂蛋白B)
可去除的蛋白质：
白蛋白
IgG
α1-抗胰酶蛋白
IgA
IgM
转铁蛋白
触珠蛋白
α2-巨球蛋白
纤维蛋白原
补体蛋白C3
α1-酸性糖蛋白 (血清类粘蛋白)
HDL (载脂蛋白A-I 和A-II)
LDL (主要是载脂蛋白B)
Legal Information
Seppro is a trademark of Merck KGaA, Darmstadt, Germany
存储类别
10 - Combustible liquids
法规信息
常规特殊物品
此项目有
Maneesh Bhargava et al.
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Chien-Lun Chen et al.
Journal of proteomics, 85, 28-43 (2013-05-02)
In this study, we evaluated the reproducibility of abundant urine protein depletion by hexapeptide-based library beads and an antibody-based affinity column using the iTRAQ technique. The antibody-based affinity-depletion approach, which proved superior, was then applied in conjunction with iTRAQ to
Inmaculada Lopez-Font et al.
Molecular neurobiology, 56(12), 8603-8616 (2019-07-11)
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Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 939, 10-16 (2013-10-05)
Human plasma is a commonly used diagnostic fluid in clinical chemistry. In-depth plasma proteomic analysis is performed to search for disease biomarkers, however the large dynamic range of protein abundance in plasma presents a substantial analytical challenge. Removal of abundant
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