推荐产品
产品名称
白介素 -6 人, recombinant, expressed in HEK 293 cells, suitable for cell culture
生物来源
human
质量水平
重组
expressed in HEK 293 cells
方案
≥98% (SDS-PAGE)
表单
lyophilized powder
效能
0.2-2 ng/mL EC50
质量
endotoxin tested
分子量
calculated mol wt 20.8 kDa (183 amino acids glycosylated protein)
包装
vial of ≥10 μg
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此商品 | G2267 | SRE0024 | G6019 |
|---|---|---|---|
| technique(s) electrophoresis: suitable | technique(s) - | technique(s) - | technique(s) - |
| grade for molecular biology | grade - | grade - | grade - |
| form powder | form lyophilized powder | form - | form lyophilized powder |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| mol wt ~36 kDa | mol wt - | mol wt - | mol wt - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
一般描述
生化/生理作用
白细胞介素-6 (IL-6) 是最初在双链 RNA 刺激的细胞培养基中发现的一种多功能蛋白。IL-6 似乎直接参与了感染和损伤后发生的反应，并可能证明在调节急性期反应方面与 IL-1 和 TNF-α 同样重要。据报道，IL-6 由成纤维细胞、活化的 T 细胞、活化的单核细胞或巨噬细胞以及内皮细胞产生。作用于多种细胞，包括成纤维细胞、髓系祖细胞、T 细胞、B 细胞和肝细胞。如许多同义词所示，IL-6 诱导多种效应：浆细胞瘤生长因子 (PCT-GF)、干扰素-β-2（IFN-β 2 ）、单核细胞来源的人 B 细胞生长因子、B 细胞刺激因子 (BSF-2)、肝细胞刺激因子 (HSF)、白细胞介素杂交瘤/浆细胞瘤-1 (IL-HP1)。此外，IL-6 似乎在 T 淋巴细胞增殖中与 IL-2 相互作用。IL-6 还可增强 IL-3 对多潜能造血祖细胞的增殖作用。
外形
该产品是以从不含添加剂或载体蛋白的pH 7.4磷酸盐缓冲液（PBS）进行冻干的冻干粉形式提供的。它是无菌填充的。
制备说明
开盖前先将小管简短离心。推荐用无菌水将蛋白重悬至终浓度为100 μg/mL。 推荐加入0.1%的重组人血清白蛋白。 产品在提供后可稳定至少2年。 在重悬后建议将蛋白按工作溶液进行分装并储存在-20°C。
分析说明
重组人IL-6生物活性的检测可通过在培养物测量其刺激IL-6依赖性小鼠T1165细胞增殖的能力而实现。
储存分类代码
11 - Combustible Solids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
K Nakashima et al.
Seminars in hematology, 35(3), 210-221 (1998-07-31)
gp130 is a common signal transducing component of the functional receptor complexes for the interleukin (IL)-6 family of cytokines, ie, IL-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M, ciliary neurotrophic factor, and cardiotrophin-1. These cytokines exhibit pleiotropic biological activities in
Janina Wolf et al.
Cytokine, 70(1), 11-20 (2014-07-06)
Interleukin-6 (IL-6) is a multifunctional cytokine with well-defined pro- and anti-inflammatory properties. Although only small amounts in the picogram range can be detected in healthy humans, IL-6 expression is highly and transiently up-regulated in nearly all pathophysiological states. IL-6 induces
Andrew D Cook et al.
Journal of immunology (Baltimore, Md. : 1950), 171(9), 4816-4823 (2003-10-22)
Many diseases are characterized by inflammatory reactions involving both the innate and adaptive arms of the immune system. Thioglycolate medium (TM) injection into the peritoneal cavity has long been used as a stimulus for eliciting inflammatory macrophages for study and
T Orita et al.
Journal of biochemistry, 115(2), 345-350 (1994-02-01)
A glycosylated form of human interleukin 6 (hIL-6) has been produced in Chinese hamster ovary (CHO) cells transfected with a cDNA clone for human IL-6. Recombinant hIL-6 was purified from a culture supernatant of the transfected CHO cells, and used
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