推荐产品
检测方案
≥95% (HPLC)
形式
lyophilized
组成
Peptide Content, ≥80%
储存条件
protect from light
储存温度
−20°C
Amino Acid Sequence
Z-Arg-Arg-pNA
一般描述
Cathepsin B, a bilobal protein, belongs to the papain-like family of cysteine proteases. It is produced as a preproenzyme. Its catalytic site is present at the interface between the two lobes. Cathepsin B has an occluding loop. It is located in the secretory vesicles of the neuronal cells. Active cathepsin B is found in the endosomal or lysosomal compartment under normal physiological conditions.
生化/生理作用
Cathepsin B is a lysosomal cysteine proteinase that metabolizes important molecules such as β-amyloid precursor protein into harmless fragments. Cathepsin B may be detected using the chromogenic substrate Z-Arg-Arg-pNA (z-arg-arg-p-nitroanalide) or flourogenic substrate Z-Arg-Arg-AMC (z-Arg-Arg-amino-4-methylcoumarin).
Cathepsin B possesses endopeptidase and exopeptidase activity. Active cathepsin B is mostly engaged in intracellular and extracellular protein turnover, which helps cells maintain homeostatic metabolic activity. It also participates in the regulation of pro-hormone and pro-enzyme activation, antigen processing, inflammatory reactions against antigens, tissue remodeling, and apoptosis. Cathepsin B plays a key role in acute pancreatitis. It also plays a vital role in lipid metabolism in atherosclerosis.
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Cathepsin B: Basis Sequence: Mouse.
Dora Cavallo-Medved et al.
The AFCS-nature molecule pages, 2011 (2011-01-01)
S Hasnain et al.
The Journal of biological chemistry, 268(1), 235-240 (1993-01-05)
The pH dependence of cathepsin B-catalyzed hydrolyzes is very complex. At least seven dissociable groups are involved in the binding and hydrolysis of 7-amido-4-methyl coumarin and p-nitroaniline (pNA)-based substrates containing a P1 Arg and either a Phe or Arg at
Yasuhito Sako et al.
Experimental parasitology, 127(3), 693-701 (2010-11-26)
Cysteine peptidases have potent activities in the pathogenesis of various parasitic infections, and are considered as targets for chemotherapy and antigens for vaccine. In this study, two cathepsin B-like cysteine peptidases (EmCBP1 and EmCBP2) from Echinococcus multilocularis metacestodes were identified
Xiaohua Peng et al.
Journal of immunology (Baltimore, Md. : 1950), 209(7), 1314-1322 (2022-09-28)
Postviral bacterial infections are a major health care challenge in coronavirus infections, including COVID-19; however, the coronavirus-specific mechanisms of increased host susceptibility to secondary infections remain unknown. In humans, coronaviruses, including SARS-CoV-2, infect lung immune cells, including alveolar macrophages, a
Ilaria Giusti et al.
Neoplasia (New York, N.Y.), 10(5), 481-488 (2008-05-14)
Vesicles shed by cancer cells are known to mediate several tumor-host interactions. Tumor microenvironment may, in turn, influence the release and the activity of tumor-shed microvesicles. In this study, we investigated the molecular mediators of the pH-dependent proinvasive activity of
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门