SBR00051
Trizma® 盐酸盐
pH 8.5, DNase and Microbial DNA free, 10 mM
别名:
TRIS 盐酸盐, 三羟基甲基氨基甲烷 盐酸盐, 三羟甲基氨基甲烷盐酸盐, 盐酸三甲胺
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线性分子式:
NH2C(CH2OH)3 · HCl
化学文摘社编号:
分子量:
157.60
NACRES:
NA.25
UNSPSC Code:
12161700
Beilstein/REAXYS Number:
3675235
MDL number:
产品名称
Trizma® 盐酸盐, pH 8.5, DNase and Microbial DNA free, 10 mM
SMILES string
Cl.NC(CO)(CO)CO
InChI
1S/C4H11NO3.ClH/c5-4(1-6,2-7)3-8;/h6-8H,1-3,5H2;1H
InChI key
QKNYBSVHEMOAJP-UHFFFAOYSA-N
concentration
10 mM
pH
8.5
useful pH range
7.0-9.0
pKa (25 °C)
8.1
storage temp.
2-8°C
Quality Level
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Other Notes
所有缓冲液的pH值都与温度和浓度有关。对于 Tris 缓冲液，温度每降低 1°C，pH 就会增加约 0.03 单位，每稀释 10 倍，pH 会降低 0.03-0.05 单位。对于一些精准的应用，请使用经过仔细校准的pH计和玻璃/甘汞组合电极。
Prepared with BioUltra Trizma Base for molecular biology in 18 megaohm UV irradiated water. pH-adjusted and 0.2 μm filtered. The buffer was tested by 35 cycles of PCR for prokaryotic and eukaryotic DNA contamination. To keep the buffer contamination-free, it is recommended to use gloves and barrier-tip pipettes for dispensing. Between uses, keep the bottle closed and refrigerated, away from areas where DNA samples and PCR products may be stored.
Application
Trizma® hydrochloride solution can be used:
- As an elution buffer after PCR cleanup
- As an elution buffer after DNA extraction
General description
10 mM Trizma® hydrochloride (pH 8.5) is a widely used buffer in molecular biology applications such as nucleic acid extraction and purification. No mixing or pH adjustment is necessary. Guaranteed accuracy for this buffer is ± 0.2 pH units at 25°C.
Preparation Note
Allow the buffer to reach room temperature before use.
Legal Information
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
12 - Non Combustible Liquids
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