Hygromycin B

抗菌剂和抗真菌剂。作用方式：在原核生物和真核生物中，通过诱导 mRNA 模板的误读而抑制蛋白质合成。它可选择性透入因病毒感染而变得可渗透的细胞。

Mode of Action: The product acts by inhibiting protein synthesis by inducing the misreading of the m-RNA template in the prokaryote, with the potency to inhibit translation.

Hygromycin B. has been used to study protein synthesis at the level of the 70S ribosome translocation and mRNA template misreadin. as an antiviral agent by selectively penetrating cells rendered permeable by virus infection and inhibiting translation and as a selection agent for hygromycin resistance gene transformed cells. It is recommended for use as a selection agent at 100-800 μg/m. specifically at 100 μg/mL for prokaryote. 200 μg/mL for lower eukaryotes and 150-400 μg/mL for higher eukaryotes

Hygromycin B, also known as Hydrovetine and D-streptamine, is a distinctive aminoglycoside antibiotic recognized for its ability to impede the growth of both prokaryotic microorganisms, such as bacteria, and eukaryotic microorganisms like yeasts and mammalian cells. Its mechanism of action involves reinforcing the interaction of peptidyl-tRNA in the ribosomal acceptor ("A") site, ultimately hindering the translocation process mediated by EF-2 (elongation Factor-2). Derived from Streptomyces hygroscopicus, this aminoglycoside antibiotic serves as a valuable selection agent for cells harboring the hygromycin-resistant gene, known as hph or hyg.

Widely utilized for selection and maintenance purposes, Hygromycin B showcases its inhibitory prowess on protein synthesis, contributing to its role in the selection and upkeep of both prokaryotic and eukaryotic cells featuring the hygromycin resistance gene. This antibiotic demonstrates anthelmintic activity in addition to its impact on translational processes in ribosomes across diverse organisms. Its specificity in inhibiting translocation, as well as its influence on decoding fidelity, makes Hygromycin B a valuable tool for the selection of transfected cells containing the Escherichia coli hygromycin resistance gene (hyg or hph) and for maintaining the Hygromycin phenotype in resistant cells. The monophasic inhibition of polypeptide synthesis suggests a single-site binding of Hygromycin B to the ribosome, adding a layer of specificity to its mode of action, making it indispensable for various facets of scientific research in cell biology and biochemical research.

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