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重组
expressed in E. coli
描述
C-Terminal six-histidine tagged
方案
≥90% (SDS-PAGE)
表单
aqueous solution
分子量
64.5 kDa
浓度
0.3 mg/mL
UniProt登记号
运输
dry ice
储存温度
−70°C
生化/生理作用
BmrA is an efflux protein and ABC transporter that acts as an ATP-dependent drug efflux pump.
制备说明
Formulated in 50mM Tris-Cl pH 8.0, 100mM NaCl, 0.01% DDM, 10% glycerol.
储存及稳定性
Thaw on ice. Upon first thaw, briefly spin tube containing protein to recover full content of the tube. Aliquot into single use aliquots. Store remaining undiluted protein in aliquots at -80°C. Note: avoid freeze/thaw cycles.
底物
MSSSHHHHHHMPTKKQKSKSKLKPFFALVRRTNPSYGKLAFALALSVVTTLVSLLIPLLTKQLVDGFSMSNLSGTQIGLIALVFFVQAGLSAYATYALNYNGQKIISGLRELLWKKLIKLPVSYFDTNASGETVSRVTNDTMVVKELITTHISGFITGIISVIGSLTILFIMNWKLTLLVLVVVPLAALILVPIGRKMFSISRETQDETARFTGLLNQILPEIRLVKASNAEDVEYGRGKMGISSLFKLGVREAKVQSLVGPLISLVLMAALVAVIGYGGMQVSSGELTAGALVAFILYLFQIIMPMGQITTFFTQLQKSIGATERMIEILAEEEEDTVTGKQIENAHLPIQLDRVSFGYKPDQLILKEVSAVIEAGKVTAIVGPSGGGKTTLFKLLERFYSPTAGTIRLGDEPVDTYSLESWREHIGYVSQESPLMSGTIRENICYGLERDVTDAEIEKAAEMAYALNFIKELPNQFDTEVGERGIMLSGGQRQRIAIARALLRNPSILMLDEATSSLDSQSEKSVQQALEVLMEGRTTIVIAHRLSTVVDADQLLFVEKGEITGRGTHHELMASHGLYRDFAEQQLKMNADLENKAG
储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
Emmanuelle Steinfels et al.
Biochemistry, 43(23), 7491-7502 (2004-06-09)
The involvement of transporters in multidrug resistance of bacteria is an increasingly challenging problem, and most of the pumps identified so far use the protonmotive gradient as the energy source. A new member of the ATP-binding cassette (ABC) family, known
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