生物来源
alpaca
重组
expressed in E. coli
偶联物
horse radish peroxidase (HRP)
抗体形式
affinity purified immunoglobulin
抗体产品类型
secondary antibodies
克隆
recombinant monoclonal
形式
lyophilized solid
分子量
14 kDa
种属反应性
human, Alpaca
技术
ELISA: 1:10000-1:50000
western blot: 1:10000-1:50000
尺寸
0.1 mL
颜色
colorless
同位素/亚型
VHH
运输
wet ice
储存温度
2-8°C
一般描述
Alpaca Anti-Human IgG(Fcγ) HRP single domain Antibody is designed to specifically detect human IgG Fcγ fragment.It is a monoclonal, single domain, recombinant antibody to human IgG Fc coupled to HRP. Based on immunoelectrophoresis and/or ELISA,Alpaca Anti-Human IgG(Fcγ) HRP single domain Antibody reacts with the Fc fragment of human IgG heavy chain but not with the Fab portion of human IgG.
In mammals, antibodies are classified into five main classes or isotypes – IgA, IgD, IgE, IgG and IgM. They are classed according to the heavy chain they contain – alpha, delta, epsilon, gamma or mu respectively. IgG is the most abundant antibody in normal human serum, accounting for 70-85% of the total immunoglobulin pool. Human IgG consists of four human subclasses (IgG1, IgG2, IgG3 and IgG4), and each contains a different heavy chain. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains(kappa or lambda). The heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kD.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(less than15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
In mammals, antibodies are classified into five main classes or isotypes – IgA, IgD, IgE, IgG and IgM. They are classed according to the heavy chain they contain – alpha, delta, epsilon, gamma or mu respectively. IgG is the most abundant antibody in normal human serum, accounting for 70-85% of the total immunoglobulin pool. Human IgG consists of four human subclasses (IgG1, IgG2, IgG3 and IgG4), and each contains a different heavy chain. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains(kappa or lambda). The heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kD.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(less than15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
特异性
Human IgG(Fcγ fragment specific)
Isotype: VHH domain of alpaca IgG2b/2c
Isotype: VHH domain of alpaca IgG2b/2c
免疫原
Recombinant Alpaca single variable domain secondary nano antibody
包装
100 μg in Glass bottle
外形
Lyophilized from 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose
重悬
Add 100uL dH2O or 100uL dH2O with 50% Glycerol to a final concentration of 1mg/mL
储存及稳定性
After reconstitution, store at –20 °C(Avoid freeze / thaw cycles). Protect from light. Stable for 12 months at -20°C
其他说明
Recognizes human IgG Fcγ fragment specifically, and reacts with cynomolgus IgG. No Cross-reactivity to rabbit , mouse, rat, goat IgG
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警示用语：
Warning
危险声明
危险分类
Aquatic Chronic 3 - Skin Sens. 1
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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