生物来源
alpaca
重组
expressed in HEK 293 cells (Human cell line)
偶联物
horse radish peroxidase (HRP)
抗体形式
affinity purified immunoglobulin
抗体产品类型
secondary antibodies
克隆
recombinant monoclonal
形式
lyophilized solid
分子量
100 kDa
种属反应性
mouse, Alpaca
技术
ELISA: 1:10000-1:50000
western blot: 1:10000-1:50000
尺寸
0.1 mL
颜色
colorless
同位素/亚型
VHH
运输
wet ice
储存温度
2-8°C
一般描述
Goat Anti-Mouse IgG2a(Fcγ) HRP Alpaca Heavy chain only Antibody is designed to specifically detect mouse IgG2a Fcγ fragment. It is a monoclonal, recombinant, goat IgG Fc fused single domain antibody to mouse IgG2a Fcγ fragment coupled to HRP. Based on immunoelectrophoresis and/or ELISA, Anti-Mouse IgG2a(Fcγ Fragment specific), AlpHcAbs Goat antibody(HRP) reacts with the Fc fragment of mouse IgG2a selectively but not with other mouse IgG subclasses, mouse IgM, or the Fab portion of mouse immunoglobulins.
Most monoclonal antibodies are generated in mouse. There are five antibody isotypes (IgA, IgD, IgE, IgG, and IgM) from mouse. Each isotype has a different heavy chain. Mouse IgG constitutes 75% of serum immunoglobulins, and IgG is the predominant form of first antibody produced from mouse. Mouse IgG consists of five subclasses-IgG1, IgG2a, IgG2b, IgG2c(inbred mouse strains with the Igh1-b allele have IgG2c isotype instead of IgG2a), IgG3. They are highly homologous and differ mainly in the hinge region. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains, and the heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kD.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(less than15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
Most monoclonal antibodies are generated in mouse. There are five antibody isotypes (IgA, IgD, IgE, IgG, and IgM) from mouse. Each isotype has a different heavy chain. Mouse IgG constitutes 75% of serum immunoglobulins, and IgG is the predominant form of first antibody produced from mouse. Mouse IgG consists of five subclasses-IgG1, IgG2a, IgG2b, IgG2c(inbred mouse strains with the Igh1-b allele have IgG2c isotype instead of IgG2a), IgG3. They are highly homologous and differ mainly in the hinge region. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains, and the heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kD.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(less than15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
特异性
Mouse IgG2a (Fcγ fragment specific)
Isotype: VHH domain of alpaca IgG2b/2c fused to goat IgG Fc
Isotype: VHH domain of alpaca IgG2b/2c fused to goat IgG Fc
免疫原
Recombinant Alpaca single variable domain secondary nano antibody
包装
100 μg in Glass bottle
外形
Lyophilized from 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose
重悬
Add 100uL dH2O or 100uL dH2O with 50% Glycerol to a final concentration of 1mg/mL
储存及稳定性
After reconstitution, store at –20 °C(Avoid freeze / thaw cycles). Protect from light. Stable for 12 months at -20°C
其他说明
No cross-reactivity with mouse IgG1/2b/3, mouse IgM, rabbit, human, cynomolgus, rat, goat IgG
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警示用语：
Warning
危险声明
危险分类
Aquatic Chronic 3 - Skin Sens. 1
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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