生物来源
alpaca
重组
expressed in E. coli
偶联物
unconjugated (6*his tag and one cys were added at the C terminal of the VHH)
抗体形式
affinity purified immunoglobulin
抗体产品类型
secondary antibodies
克隆
recombinant monoclonal
形式
lyophilized solid
分子量
14 kDa
种属反应性
mouse, Alpaca
技术
ELISA: 1:5000-1:20000
western blot: 1:5000-1:20000
尺寸
0.1 mL
颜色
colorless
同位素/亚型
VHH
运输
wet ice
储存温度
2-8°C
一般描述
Alpaca Anti-Mouse kappa single domain Antibody is designed to specifically detect mouse IgG kappa chain. It is a monovalent, single domain, recombinant antibody derived from the variable region of heavy chain of Alpaca pacous. Based on immunoelectrophoresis and/or ELISA, Alpaca Anti-Mouse kappa single domain Antibody reacts with the mouse IgG kappa chain selectively but not with the mouse IgG lambda chain or heavy chain of mouse IgG.
Most monoclonal antibodies are generated in mouse. There are five antibody isotypes (IgA, IgD, IgE, IgG, and IgM) from mouse. Each isotype has a different heavy chain. Mouse IgG constitutes 75% of serum immunoglobulins, and IgG is the predominant form of first antibody produced from mouse. Mouse IgG consists of five subclasses-IgG1, IgG2a, IgG2b, IgG2c(inbred mouse strains with the Igh1-b allele have IgG2c isotype instead of IgG2a), IgG3. They are highly homologous and differ mainly in the hinge region. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains(kappa or lambda), and the heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kD.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
Most monoclonal antibodies are generated in mouse. There are five antibody isotypes (IgA, IgD, IgE, IgG, and IgM) from mouse. Each isotype has a different heavy chain. Mouse IgG constitutes 75% of serum immunoglobulins, and IgG is the predominant form of first antibody produced from mouse. Mouse IgG consists of five subclasses-IgG1, IgG2a, IgG2b, IgG2c(inbred mouse strains with the Igh1-b allele have IgG2c isotype instead of IgG2a), IgG3. They are highly homologous and differ mainly in the hinge region. The whole IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains(kappa or lambda), and the heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kD.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small(<15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.
特异性
Mouse IgG kappa chain
Isotype: VHH domain of alpaca IgG2b/2c
Isotype: VHH domain of alpaca IgG2b/2c
免疫原
Recombinant Alpaca single variable domain secondary nano antibody
包装
100 μg in Glass bottle
外形
Lyophilized from 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose
重悬
Add 100uL dH2O or 100uL dH2O with 50% Glycerol to a final concentration of 1mg/mL
储存及稳定性
After reconstitution, store at –20 °C(Avoid freeze / thaw cycles). Stable for 12 months at -20°C
其他说明
No cross-reactivity with rabbit, human, cynomolgus, rat, goat IgG
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警示用语：
Warning
危险声明
危险分类
Aquatic Chronic 3 - Skin Sens. 1
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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