跳转至内容
Merck
CN

SAB4200853

Sigma-Aldrich

Anti-PBP2a of MRSA antibody, Mouse monoclonal

clone 38, purified from hybridoma cell culture

别名:

Anti-Beta-lactam-inducible penicillin-binding protein 2a

登录查看公司和协议定价


About This Item

UNSPSC代码:
12352203
NACRES:
NA.43

抗体形式

purified from hybridoma cell culture

质量水平

抗体产品类型

primary antibodies

克隆

38, monoclonal

表单

liquid

浓度

~1 mg/mL

技术

immunoblotting: 0.5-1 μg/mL using whole recombinant PBP2a protein

同位素/亚型

IgG1

运输

dry ice

储存温度

−20°C

靶向翻译后修饰

unmodified

一般描述

Infections caused by methicillin-resistant Staphylococcus aureus (MRSA) are a major concern cause for clinicians, presenting high mortality and morbidity rates compared to infections caused by methicillin-sensitive Staphylococci.2

特异性

Monoclonal Anti-PBP2a of MRSA antibody specifically recognizes PBP2a protein from Methicillin-resistant Staphylococcus aureus (MRSA). The antibody reacts with both denaturated recombinant PBP2a protein and bacteria lysates in Immunoblot.1 The antibody recognizes PBP2a in MRSA cells in both the exponential and stationary phases, however no cross reaction with methicillin-sensitive Staphylococcus aureus (PBP2a negative) and E. coli were seen.1

应用

The antibody may be used in various immunochemical techniques including Immunoblotting1 (~76 kDa), ELISA1, Surface Plasmon Resonance (SPR)1 and Flow cytometry1.

生化/生理作用

Resistance of S. aureus to methicillin and all beta-lactam antibiotics is due to the activity of PBP2a protein (i.e., penicillin binding protein2a) that is located in the cell wall of the resistant strains.3-4 PBP2a is encoded by mecA gene and belongs to a family of penicillin binding proteins (PBPs). PBPs are membrane-bound enzymes that catalyze the transpeptidation reaction necessary for cross-linkage of peptidoglycan chains.5-6 Contrary to all PBPs, PBP2a has low affinity for all ß-lactam antibiotics.7 Therefore, in the presence of ß-lactam antibiotics, while normal PBPs are blocked, the PBP2a is able to continue catalyze the transpeptidation reactions enabling cell wall synthesis.4 PBP2a protein is suggested to be a promising candidate for anti-MRSA vaccine development.8

外形

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.Antibody Concentration: ~ 1.0 mg/mL

储存及稳定性

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

免责声明

Unless otherwise stated in our catalog  our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

未找到合适的产品?  

试试我们的产品选型工具.

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

从最新的版本中选择一种:

分析证书(COA)

Lot/Batch Number

没有发现合适的版本?

如果您需要特殊版本,可通过批号或批次号查找具体证书。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门